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인용 및 참조 문헌 (10)
Invitrogen™
BioPrime™ DNA Labeling System
BioPrime™ DNA Labeling System은 biotinylated probe 준비에 사용하도록 만들어졌습니다. Random primer (octamers)를 변성된 DNA template에 anealing하고 biotin-14-dCTP가 있는 상태에서 Klenow자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 18094011 | 30 Reactions |
카탈로그 번호 18094011
제품 가격(KRW)
855,000
Each
수량:
30 Reactions
제품 가격(KRW)
855,000
Each
BioPrime™ DNA Labeling System은 biotinylated probe 준비에 사용하도록 만들어졌습니다. Random primer (octamers)를 변성된 DNA template에 anealing하고 biotin-14-dCTP가 있는 상태에서 Klenow fragment로 확장하여 DNA와 RNA의 비-방사성 검출에 사용하는 민감한 biotinylated-DNA probe를 만듭니다. BioPrime™ DNA Labeling System에서는 상당한 net DNA 합성으로 probe가 10–40배 증폭됩니다. 이 제품은 DNA양이 제한된 상황에서 특히 적합합니다. 이 제품으로 만든 probe는 Southern/northern blot, 플라크 및 콜로니의 hybridization, chromosom spread의 in situ hybridization에 사용되고 있습니다. BioPrime™ DNA Labeling System:
• Nick translation보다 필요한 template DNA 양이 적습니다.
• Template를 증폭하여 biotinylated probe 제공량을 증가시킵니다.
• 한 번 반응에 50 ∼500 ng template DNA를 표지합니다.
• Nick translation보다 필요한 template DNA 양이 적습니다.
• Template를 증폭하여 biotinylated probe 제공량을 증가시킵니다.
• 한 번 반응에 50 ∼500 ng template DNA를 표지합니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
라벨 또는 염료 포함Yes
라벨링 방법Direct Labeling
제품라인BioPrime
제품 유형DNA Labeling System
수량30 Reactions
배송 조건Approved for shipment on Wet or Dry Ice
검출 방법Biotin-based
최종 제품 유형Probes (Labeled DNA)
Labeling TargetDNA (General), Genomic DNA
라벨 또는 염료Biotin
Unit SizeEach
구성 및 보관
• 700 μl 2.5X Random Primers Solution
• 175 μl 10X dNTP Mixture
• 100 μg Control DNA
• 35 μl Klenow Fragment (40 U/μl)
• 500 μl Stop Buffer
• 1.25 ml Distilled Water
Store at -20°C in a non-frost-free freezer.
• 175 μl 10X dNTP Mixture
• 100 μg Control DNA
• 35 μl Klenow Fragment (40 U/μl)
• 500 μl Stop Buffer
• 1.25 ml Distilled Water
Store at -20°C in a non-frost-free freezer.
자주 묻는 질문(FAQ)
Can exo-Klenow be purchased separately from your array labeling kits?
인용 및 참조 문헌 (10)
인용 및 참조 문헌
Abstract
A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridization.
Journal:Genome Res
PubMed ID:14707179
'Microarray-based comparative genomic hybridization (CGH) has become a powerful method for the genome-wide detection of chromosomal imbalances. Although BAC microarrays have been used for mouse CGH studies, the resolving power of these analyses was limited because high-density whole-genome mouse BAC microarrays were not available. We therefore developed a mouse BAC
DNA replication origins fire stochastically in fission yeast.
Journal:Mol Biol Cell
PubMed ID:16251353
'DNA replication initiates at discrete origins along eukaryotic chromosomes. However, in most organisms, origin firing is not efficient; a specific origin will fire in some but not all cell cycles. This observation raises the question of how individual origins are selected to fire and whether origin firing is globally coordinated
A virulence and antimicrobial resistance DNA microarray detects a high frequency of virulence genes in Escherichia coli isolates from Great Lakes recreational waters.
Journal:Appl Environ Microbiol
PubMed ID:16751532
'Escherichia coli is generally described as a commensal species with occasional pathogenic strains. Due to technological limitations, there is currently little information concerning the prevalence of pathogenic E. coli strains in the environment. For the first time, using a DNA microarray capable of detecting all currently described virulence genes and
Microarray deacetylation maps determine genome-wide functions for yeast histone deacetylases.
Journal:Cell
PubMed ID:12086601
'Yeast contains a family of five related histone deacetylases (HDACs) whose functions are known at few genes. Therefore, we used chromatin immunoprecipitation and intergenic microarrays to generate genome-wide HDAC enzyme activity maps. Rpd3 and Hda1 deacetylate mainly distinct promoters and gene classes where they are recruited largely by novel mechanisms.
Characterization of the genome composition of Bartonella koehlerae by microarray comparative genomic hybridization profiling.
Journal:J Bacteriol
PubMed ID:16109957
Bartonella henselae is present in a wide range of wild and domestic feline hosts and causes cat-scratch disease and bacillary angiomatosis in humans. We have estimated here the gene content of Bartonella koehlerae, a novel species isolated from cats that was recently identified as an agent of human endocarditis. The