Random Primers DNA Labeling System은 DNA, 특히 fragment ––[ α-32 P]-dCTP를 사용하여 >109 cpm/μg control DNA 획득–한 번 반응에 25자세히 알아보기
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카탈로그 번호
수량
18187013
30 Reactions
카탈로그 번호 18187013
제품 가격(KRW)
491,000
Online offer
Ends: 31-Mar-2026
599,000
할인액 108,000 (18%)
Each
카트에 추가하기
수량:
30 Reactions
제품 가격(KRW)
491,000
Online offer
Ends: 31-Mar-2026
599,000
할인액 108,000 (18%)
Each
카트에 추가하기
Random Primers DNA Labeling System은 DNA, 특히 fragment <1 kb의 방사성 라벨링에 이상적입니다. Random Primers DNA Labeling System: ––[ α-32 P]-dCTP를 사용하여 >109 cpm/μg control DNA 획득 –한 번 반응에 25 ng DNA
성능 및 품질 검사: Random primer 라벨링 반응에서 대조 DNA를 사용해 방사선 표지된 뉴클리오티드의 통합을 검증합니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Radioactive
최종 제품 유형Probes (Labeled DNA)
라벨 또는 염료 포함No
라벨링 방법Direct Labeling
라벨링 타겟DNA (General)
라벨 또는 염료32P (Phosphorous-32)
제품 유형DNA Labeling System
수량30 Reactions
배송 조건Approved for shipment on Wet or Dry Ice
Unit SizeEach
구성 및 보관
Components of the Random Primers DNA Labeling System are listed in the table to the right. Store at -20°C.
자주 묻는 질문(FAQ)
How can I label COT-1 DNA?
Probes can be labeled with 32P by random primer or nick translation procedures using the Random Primers DNA Labeling System (Cat. No.18187-013) or Nick Translation System (Cat. No. 18160-010). Biotinylated COT-1 DNA can be prepared by nick translation with the BioNick Labeling System (Cat. No. 18247-015) or by the BioPrime DNA Labeling System (Cat. No. 18094-011). Improved results can be obtained when the COT-1 DNA is first ligated to itself to provide an optimum template.
인용 및 참조 문헌 (12)
인용 및 참조 문헌
Abstract
Human biliverdin reductase is a leucine zipper-like DNA-binding protein and functions in transcriptional activation of heme oxygenase-1 by oxidative stress.
Human biliverdin reductase (hBVR) is a serine/threonine kinase that catalyzes reduction of the heme oxygenase (HO) activity product, biliverdin, to bilirubin. A domain of biliverdin reductase (BVR) has primary structural features that resemble leucine zipper proteins. A heptad repeat of five leucines (L(1)--L(5)), a basic domain, and a conserved alanine ... More
Activation of the androgen receptor N-terminal domain by interleukin-6 via MAPK and STAT3 signal transduction pathways.
'The androgen receptor (AR) is a ligand-activated transcription factor that mediates the biological responses of androgens. However, non-androgenic pathways have also been shown to activate the AR. The mechanism of cross-talk between the interleukin-6 (IL-6) and AR signal transduction pathways was investigated in LNCaP human prostate cancer cells. IL-6 induced ... More
PPARgamma knockdown by engineered transcription factors: exogenous PPARgamma2 but not PPARgamma1 reactivates adipogenesis.
Authors: Ren Delin; Collingwood Trevor N; Rebar Edward J; Wolffe Alan P; Camp Heidi S;
Journal:Genes Dev
PubMed ID:11782439
'To determine functional differences between the two splice variants of PPARgamma (gamma1 and gamma2), we sought to selectively repress gamma2 expression by targeting engineered zinc finger repressor proteins (ZFPs) to the gamma2-specific promoter, P2. In 3T3-L1 cells, expression of ZFP55 resulted in >50% reduction in gamma2 expression but had no ... More
Interaction of the Poliovirus Receptor CD155 with the Dynein Light Chain Tctex-1 and Its Implication for Poliovirus Pathogenesis.
Authors: Mueller Steffen; Cao Xuemei; Welker Reinhold; Wimmer Eckard;
Journal:J Biol Chem
PubMed ID:11751937
'The cellular receptor for poliovirus CD155 (or PVR) is the founding member of a new class of membrane-associated immunoglobulin-like proteins, which include the mouse tumor-associated antigen E4 (Tage4) and three proteins termed ' ... More
A trypanosome mitochondrial RNA polymerase is required for transcription and replication.
Authors: Grams Jayleen; Morris James C; Drew Mark E; Wang Zefeng; Englund Paul T; Hajduk Stephen L;
Journal:J Biol Chem
PubMed ID:11859084
'Understanding mitochondrial transcription is a requisite first step toward understanding the regulation of mitochondrial gene expression in kinetoplastids. Here we report the identification and functional characterization of a mitochondrial RNA polymerase (mtRNAP) from Trypanosoma brucei, the first trans-acting factor involved in kinetoplast mitochondrial transcription to be identified. Using sequences conserved ... More