Gibco™ 4% Agarose는 플라크 분석과 baculovirus 정제용 가압멸균된 고순도 겔로 녹는점이 낮습니다. 4% Agarose Gel은 65 – 70°C에서 녹으며 37°C에서 액체 상태입니다. 농축된 곤충 세포 배양 배지에 희석했을 때 Gibco™ 4% Agarose는 국소적으로 감염된 부위를 고정시킬 수 있는 정도로 단단하면서도 잡아 당기면 늘어나 clonal expansion 시 플라크를 효율적으로 제거할 수 있는 겔을 생성합니다.
용도 이 제품은 연구용으로만 사용할 수 있습니다. 진단 절차에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Mammalian Cells
농도0.04
용도(애플리케이션)Plaque Assay
수량40 mL
비고Not for electrophoresis
유통 기한12 Months
배송 조건Room Temperature
형태Gel
제품 유형Agarose
멸균Sterile
Sterilization MethodAutoclaved
Unit SizeEach
구성 및 보관
Storage conditions: 2 – 8°C. Protect from light. Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
자주 묻는 질문(FAQ)
Can I autoclave agarose to prepare agarose gels?
No. Autoclaving at temperatures that are too high or pressures that are too high can hydrolyze the agarose polymer and weaken it.
How can generation of replication competent adenoviruses be avoided when using your pSilencer adeno 1.0-CMV System?
Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
For Agarose (4%) (Cat. No. 18300012), what is the agarose reconstituted in?