Search
인용 및 참조 문헌 (6)
Invitrogen™
RadPrime DNA Labeling System
The RadPrime DNA Labeling System is ideal for rapid preparation (under 10 min) of high-specific activity 32 P-labeled probes for자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 18428011 | 30 Reactions |
카탈로그 번호 18428011
제품 가격(KRW)
577,000
Online offer
Ends: 31-Dec-2025
641,000할인액 64,000 (10%)
Each
수량:
30 Reactions
제품 가격(KRW)
577,000
Online offer
Ends: 31-Dec-2025
641,000할인액 64,000 (10%)
Each
The RadPrime DNA Labeling System is ideal for rapid preparation (under 10 min) of high-specific activity 32 P-labeled probes for the detection of DNA and RNA (1). The RadPrime DNA Labeling System:
• Produces probes that can detect nucleic acids on northern or Southern blots, plaque lifts, colony hybridizations, and in situ hybridization
• Yields >109 cpm/μg control DNA using [32P]-dCTP
• Labels 25 ng of DNA in one reaction
Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verified using control DNA in a RadPrime labeling reaction.
• Produces probes that can detect nucleic acids on northern or Southern blots, plaque lifts, colony hybridizations, and in situ hybridization
• Yields >109 cpm/μg control DNA using [32P]-dCTP
• Labels 25 ng of DNA in one reaction
Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verified using control DNA in a RadPrime labeling reaction.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Radioactive
최종 제품 유형Probes (Labeled DNA)
라벨 또는 염료 포함No
라벨링 방법Direct Labeling
라벨링 타겟DNA (General)
라벨 또는 염료32P (Phosphorous-32)
제품 유형DNA Labeling System
수량30 Reactions
배송 조건Dry Ice
Unit SizeEach
구성 및 보관
Components of the RadPrime DNA Labeling System are listed in the table to the right. Store at -20°C.
.
.
자주 묻는 질문(FAQ)
How long does the labeling take when using the RadPrime DNA Labeling system?
인용 및 참조 문헌 (6)
인용 및 참조 문헌
Abstract
Molecular characterization of the homo-phytochelatin synthase of soybean Glycine max: relation to phytochelatin synthase.
Journal:J Biol Chem
PubMed ID:11706029
'The phytochelatin homologs homo-phytochelatins are heavy metal-binding peptides present in many legumes. To study the biosynthesis of these compounds, we have isolated and functionally expressed a cDNA GmhPCS1 encoding homo-phytochelatin synthase from Glycine max, a plant known to accumulate homo-phytochelatins rather than phytochelatins upon the exposure to heavy metals. The
Two Proteins Essential for Apolipoprotein B mRNA Editing Are Expressed from a Single Gene through Alternative Splicing.
Journal:J Biol Chem
PubMed ID:11815617
'Apolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A
Activation of the phosphatidylinositol 3-kinase/Akt signaling pathway by retinoic acid is required for neural differentiation of SH-SY5Y human neuroblastoma cells.
Journal:J Biol Chem
PubMed ID:12000752
'Retinoic acid (RA) induces neural differentiation of SH-SY5Y neuroblastoma cells. We show that the mRNA levels of the differentiation-inhibiting basic helix-loop-helix transcription factors ID1, ID2, and ID3 are down-regulated during RA-induced differentiation of SH-SY5Y cells. The levels of ID proteins decreased in parallel to the observed transcriptional repression. The expression
Novel Alternative Splicings of BPAG1 (Bullous Pemphigoid Antigen 1) Including the Domain Structure Closely Related to MACF (Microtubule Actin Cross-linking Factor).
Journal:J Biol Chem
PubMed ID:11751855
'BPAG1 (bullous pemphigoid antigen 1) was originally identified as a 230-kDa hemidesmosomal protein and belongs to the plakin family, because it consists of a plakin domain, a coiled-coil rod domain and a COOH-terminal intermediate filament binding domain. To date, alternatively spliced products of BPAG1, BPAG1e, and BPAG1n are known. BPAG1e
A DEAD-Box Protein Functions as an ATP-Dependent RNA Chaperone in Group I Intron Splicing.
Journal:Cell
PubMed ID:12086675
The Neurospora crassa CYT-18 protein, the mitochondrial tyrosyl-tRNA synthetase, functions in splicing group I introns by inducing formation of the catalytically active RNA structure. Here, we identified a DEAD-box protein (CYT-19) that functions in concert with CYT-18 to promote group I intron splicing in vivo and vitro. CYT-19 does not