CaptureSelect™ Human Albumin Affinity Matrix

CaptureSelect Human Albumin Affinity Matrix has been specifically designed for the purification of human albumin and albumin fusion proteins.

Features of this affinity matrix include:

• Highly selective for human albumin
• Unique elution characteristics allowing for gentle elution of albumin fusion proteins
• Excellent scalability
• Non-animal-derived

The high selectivity and yields obtained using CaptureSelect Human Albumin Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step. The mild elution conditions make the resin suitable for the purification of albumin fusion proteins, especially those that are pH sensitive.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main Characteristics:

• Matrix: agarose-based, aldehyde-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect HSA affinity ligand
• Ligand coupling method: aldehyde coupling via NH₂ residues of the ligand
• Binding capacity: ∼15 mg albumin/mL resin
• Elution conditions (neutral pH): 20 mM Tris, pH 7.4 with 2 M MgCl₂ or with 1 M NaCl + 50%(v/v) propylene glycol or with 1 M NaCl + 0.5 M L-arginine
• Elution conditions (acidic pH): 0.1 M glycine, pH 3.0
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20%(v/v) ethanol