CaptureSelect™ Prothrombin Affinity Matrix

CaptureSelect Prothrombin Affinity Matrix has been specifically designed for the purification of human prothrombin (coagulation Factor II). This matrix binds both native and recombinant human prothrombin.

Prothrombin is a vitamin-K dependent plasma protein which is produced in the liver. The presence of calcium promotes the binding of prothrombin to phospholipid bilayers.

Features of this affinity matrix include:

• Highly selective for human prothrombin without cross reactivity to other human plasma proteins
• Binds different forms of prothrombin (Factor II) in the presence of calcium
• Enables efficient purification of prothrombin with high yields and retained activity
• Mild elution at neutral pH using EDTA
• Excellent scalability
• Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: agarose-based, epoxide activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect prothrombin affinity ligand
• Ligand coupling method: epoxide coupling
• Binding capacity: estimated at > 5 mg/mL
• Binding conditions: 50 mM Tris, 150 mM NaCl, 5 mM CaCl₂, pH 7
• Elution conditions: 50 mM Tris, 150 mM NaCl, 5 mM EDTA, pH 7
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol