Pierce™ p-Aminophenyl Phosphoryl Choline Agarose

Thermo Scientific Pierce p-Amino Phosphoryl Choline Agarose enables purification of C-reactive protein (CRP).

Features of p-Amino Phosphoryl Choline Agarose:

• Ligand: p-aminophenyl phosphoryl choline
• Support: crosslinked 6% beaded agarose
• Binding capacity: greater than 3 mg of human C-reactive protein per milliliter of settled resin
• Format: 50% resin slurry in 0.02% sodium azide
• Binding condition: Tris or borate-buffered saline (pH 8 to 8.5) with 1 to 2 mM calcium chloride
• Elution condition: Tris or borate-buffered saline (pH 8 to 8.5) with 2 mM EDTA
• Reusable: resin can be regenerated and reused several times

The immobilized phosphoryl choline is a beaded agarose affinity resin for purification of C-reactive protein (CRP) from plasma, ascites and other biological fluids. CRP is a pentameric (pentraxin) protein that is classified as an acute phase reactant because of its high level of production during inflammation. The protein binds to phosphoryl choline groups on the surface of microbes and has been linked to several biological functions including activation of the classical complement pathway, enhancement of phagocytosis and interaction with certain subpopulations of T-lymphocytes. In the late 1970s, Volanakis and colleagues discovered a method of isolating and studying C-reactive protein by using the protein's affinity for phosphoryl choline that has been immobilized to a porous solid chromatography support.