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인용 및 참조 문헌 (3)
Gibco™
Basal Medium Eagle (BME)
Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells.자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 21010046 | 500 mL |
카탈로그 번호 21010046
제품 가격(KRW)
63,000
Online offer
Ends: 31-Dec-2025
66,000할인액 3,000 (5%)
Each
수량:
500 mL
제품 가격(KRW)
63,000
Online offer
Ends: 31-Dec-2025
66,000할인액 3,000 (5%)
Each
Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.
This BME is manufactured as follows:
The complete formulation is available.
BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
Dual-Site cGMP Manufacturing
Gibco™ BME is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ BME product made in our Scotland facility (410100-026). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
This product is not used for in vitro diagnostic purpose in some countries.
This BME is manufactured as follows:
| With | Without |
| • Phenol Red | • L-glutamine |
| • HEPES |
The complete formulation is available.
BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
Dual-Site cGMP Manufacturing
Gibco™ BME is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ BME product made in our Scotland facility (410100-026). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
This product is not used for in vitro diagnostic purpose in some countries.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포주HeLa, mouse fibroblasts, WI-38 and MRC-5
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형Basal Medium Eagle (BME)
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Liquid
멸균Sterile-filtered
첨가제 포함Low Glucose, Phenol Red
첨가제 없음No Glutamine, No HEPES, No Sodium Pyruvate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
자주 묻는 질문(FAQ)
How long can I keep my media after supplementing with serum?
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
How can I remove mycoplasma contamination from my cell culture medium?
I see a decrease in growth of my culture. What should I do?
My cells are not adhering to the culture vessel. What should I do?
인용 및 참조 문헌 (3)
인용 및 참조 문헌
Abstract
Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferation.
Journal:J Biol Chem
PubMed ID:11821386
'MENT (Myeloid and Erythroid Nuclear Termination stage-specific protein) is a developmentally regulated chromosomal serpin that condenses chromatin in terminally differentiated avian blood cells. We show that MENT is an effective inhibitor of the papain-like cysteine proteinases cathepsins L and V. In addition, ectopic expression of MENT in mammalian cells is
Sulfation in the Golgi lumen of Madin-Darby canine kidney cells is inhibited by brefeldin A and depends on a factor present in the cytoplasm and on Golgi membranes.
Journal:J Biol Chem
PubMed ID:12138122
'Madin-Darby canine kidney cells are more resistant than most other cell types to the classical effects of brefeldin A (BFA) treatment, the induction of retrograde transport of Golgi cisternae components to the endoplasmic reticulum. Here we show that sulfation of heparan sulfate proteoglycans (HSPGs), chondroitin sulfate proteoglycans (CSPGs), and proteins
Pituitary Adenylyl Cyclase-activating Polypeptide Prevents Induced Cell Death in Retinal Tissue through Activation of Cyclic AMP-dependent Protein Kinase.
Journal:J Biol Chem
PubMed ID:11847214
Multiple neuroactive substances are secreted by neurons and/or glial cells and modulate the sensitivity to cell death. In the developing retina, it has been shown that increased intracellular levels of cAMP protect cells from degeneration. We tested the hypothesis that the neuroactive peptide pituitary adenylyl cyclase-activating polypeptide (PACAP) has neuroprotective