DMEM/F-12, no phenol red

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인용 및 참조 문헌 (1)

Gibco™

DMEM/F-12, no phenol red

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different자세히 알아보기

카탈로그 번호	수량
21041025	500 mL

카탈로그 번호 21041025

제품 가격(KRW)

79,000

온라인 행사

Ends: 31-Dec-2025

83,000

할인액 4,000 (5%)

카트에 추가하기

500 mL

Customize this product

제품 가격(KRW)

79,000

온라인 행사

Ends: 31-Dec-2025

83,000

할인액 4,000 (5%)

카트에 추가하기

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

With	Without
• L-glutamine	• HEPES
	• Phenol Red

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

For Research Use Only. Not for use in diagnostic procedures.

세포주MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts

농도1 X

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco

제품 유형DMEM (Dulbecco's Modified Eagle Medium)/F-12

수량500 mL

유통 기한12 Months From Date of Manufacture

배송 조건Room Temperature

분류Animal Origin-free

형태Liquid

Serum LevelStandard Serum Supplementation

멸균Sterile-filtered

첨가제 포함High Glucose, Glutamine, Sodium Pyruvate

첨가제 없음No Phenol Red, No HEPES

Unit SizeEach

구성 및 보관

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all DMEM/F-12, no phenol red FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌

Abstract

Study of neurotoxic intracellular calcium signalling triggered by amyloids.

Authors:Villalobos C, Caballero E, Sanz-Blasco S, Núñez L

Journal:Methods Mol Biol

PubMed ID:22528098

Neurotoxicity in Alzheimer's disease (AD) is associated to dishomeostasis of intracellular Ca(2+) induced by amyloid ß peptide (Aß) species. Understanding of the effects of Aß on intracellular Ca(2+) homeostasis requires preparation of the different Aß assemblies including oligomers and fibrils and the testing of their effects on cytosolic and mitochondrial ... More