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IMDM, no phenol red
IMDM, no phenol red
인용 및 참조 문헌 (3)
Gibco™

IMDM, no phenol red

Iscove's Modified Dulbecco's Medium (IMDM)은 Jurkat, COS-7, 대식세포 등 빠른 증식과 고밀도 배양이 필요한 세포주에 적합합니다. Gibco™은 다양한 용도의 IMDM자세히 알아보기
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카탈로그 번호수량
21056023500 mL
카탈로그 번호 21056023
제품 가격(KRW)
94,000
온라인 행사
Ends: 31-Mar-2026
104,000
할인액 10,000 (10%)
Each
카트에 추가하기
수량:
500 mL
Customize this product
제품 가격(KRW)
94,000
온라인 행사
Ends: 31-Mar-2026
104,000
할인액 10,000 (10%)
Each
카트에 추가하기

Iscove's Modified Dulbecco's Medium (IMDM)은 Jurkat, COS-7, 대식세포 등 빠른 증식과 고밀도 배양이 필요한 세포주에 적합합니다. Gibco™은 다양한 용도의 IMDM 변형 제품을 제공합니다. Media Selector Tool (미디어 선택 도구)를 통해 귀하의 배양 조건에 가장 적합한 조합을 찾을 수 있습니다.

본 IMDM은 아래와 같이 변형된 조성을 가집니다:

포함: L-glutamine

불포함: Phenol Red, α-thioglycerol, 2-mercaptoethanol

전체 조성표는 상세 페이지에서 확인 가능합니다.

본 IMDM은 Dulbecco’s Modified Eagle Medium (DMEM)을 기반으로 하며, 셀레늄 및 추가 아미노산, 비타민을 포함합니다. 철 성분은 제외되었고, 질산칼륨이 질산철을 대신합니다.

사용 목적 및 안내:
연구용으로만 사용 가능하며, 동물 또는 사람의 진단/치료 목적으로는 사용되지 않습니다.

IMDM에는 단백질, 지질, 성장인자가 들어있지 않습니다. 따라서 본 배지를 사용하려면 일반적으로 Fetal Bovine Serum (FBS) 10%를 보충하여 사용합니다. 탄산수소나트륨 버퍼 시스템(3.024 g/L)을 사용하므로 생리적 pH 유지를 위해 CO2 5-10% 환경이 필요합니다.

For Research Use Only. Not for use in diagnostic procedures.

사양
세포주Jurkat, COS-7, and macrophage cells
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형IMDM (Iscove's Modified Dulbecco's Medium)
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Liquid
멸균Sterile-filtered
첨가제 포함High Glucose, Glutamine, HEPES, Sodium Pyruvate
첨가제 없음No Phenol Red, No α-Thioglycerol, No 2-Mercaptoethanol
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all IMDM, no phenol red FAQs

인용 및 참조 문헌 (3)

인용 및 참조 문헌
Abstract
Nongenomic testosterone calcium signaling. Genotropic actions in androgen receptor-free macrophages.
Authors:Guo Z, Benten WP, Krucken J, Wunderlich F
Journal:J Biol Chem
PubMed ID:12048191
'Steroid hormones exert genotropic actions through members of the nuclear receptor family. Here, we have demonstrated genotropic actions of testosterone that are independent of intracellular androgen receptors (iAR). Through plasma membrane androgen receptors (mAR), testosterone induces a rapid rise in the intracellular free Ca(2+) concentration of iAR-free murine RAW 264.7 ... More
The PAX3-FKHR fusion protein created by the t(2;13) translocation in alveolar rhabdomyosarcomas is a more potent transcriptional activator than PAX3.
Authors:Fredericks WJ, Galili N, Mukhopadhyay S, Rovera G, Bennicelli J, Barr FG, Rauscher FJ 3rd
Journal:Mol Cell Biol
PubMed ID:7862145
Alveolar rhabdomyosarcomas are pediatric solid tumors with a hallmark cytogenetic abnormality: translocation of chromosomes 2 and 13 [t(2;13) (q35;q14)]. The genes on each chromosome involved in this translocation have been identified as the transcription factor-encoding genes PAX3 and FKHR. The NH2-terminal paired box and homeodomain DNA-binding domains of PAX3 are ... More
Role of JunB in erythroid differentiation.
Authors: Jacobs-Helber Sarah M; Abutin Randolph M; Tian Cuixia; Bondurant Maurice; Wickrema Amittha; Sawyer Stephen T;
Journal:J Biol Chem
PubMed ID:11726656
The role of junB as a regulator of erythroid cell survival, proliferation, and differentiation was tested by controlled expression of JunB in the erythropoietin (EPO)-dependent erythroleukemia cell line HCD57. JunB induced erythroid differentiation as evidenced by increased expression of the erythroid-specific proteins beta-globin, spectrin-alpha, and TER-119. Expression of JunB for ... More