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인용 및 참조 문헌 (2)
Gibco™
DMEM, high glucose, HEPES, no phenol red
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 수량 |
|---|---|
| 21063029 | 500 mL |
| 21063045 | 10 x 500 mL |
카탈로그 번호 21063029
제품 가격(KRW)
112,000
キャンペーン価格
Ends: 31-Dec-2025
117,000할인액 5,000 (4%)
Each
수량:
500 mL
제품 가격(KRW)
112,000
キャンペーン価格
Ends: 31-Dec-2025
117,000할인액 5,000 (4%)
Each
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
This DMEM is modified as follows:
| With | Without |
| • High Glucose | • Sodium Pyruvate |
| • L-glutamine | • Phenol Red |
| • HEPES |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포주HeLa, 293, Cos-7, and PC-12
세포 유형Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
농도1 X
Glucose 농도4500 mg/L
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형DMEM (Dulbecco's Modified Eagle Medium)
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Liquid
Serum LevelStandard Serum Supplementation
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함High Glucose, Glutamine, HEPES
첨가제 없음No Phenol Red, No Sodium Pyruvate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
자주 묻는 질문(FAQ)
My cells will not grow in DMEM, what other type of culture media can be used with the Photoreactive Amino Acids?
What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?
I understand that some media are worse than others for fluorescence imaging. How do I choose?
Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?
How long can I keep my media after supplementing with serum?
인용 및 참조 문헌 (2)
인용 및 참조 문헌
Abstract
Basolateral membrane expression of a K+ channel, Kir 2.3, is directed by a cytoplasmic COOH-terminal domain.
Journal:Proc Natl Acad Sci U S A
PubMed ID:11504929
'The inwardly rectifying potassium channel Kir 2.3 is specifically targeted and expressed on the basolateral membrane of certain renal epithelial cells. In the present study, the structural basis for polarized targeting was elucidated. Deletion of a unique COOH-terminal domain produced channels that were mistargeted to the apical membrane, consistent with
Identification of novel compounds that increase SMN protein levels using an improved SMN2 reporter cell assay.
Journal:J Biomol Screen
PubMed ID:22233647
Spinal muscular atrophy (SMA) is a neurodegenerative disorder that is characterized by progressive loss of motor neuron function. It is caused by the homozygous loss of the SMN1 (survival of motor neuron 1) gene and a decrease in full-length SMN protein. SMN2 is a nearly identical homolog of SMN1 that,