DMEM, high glucose, no glutamine, no calcium
DMEM, high glucose, no glutamine, no calcium
인용 및 참조 문헌 (2)
Gibco™

DMEM, high glucose, no glutamine, no calcium

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.자세히 알아보기
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카탈로그 번호수량
21068028500 mL
카탈로그 번호 21068028
제품 가격(KRW)
176,000
キャンペーン価格
Ends: 31-Dec-2025
185,000
할인액 9,000 (5%)
Each
카트에 추가하기
수량:
500 mL
Customize this product
제품 가격(KRW)
176,000
キャンペーン価格
Ends: 31-Dec-2025
185,000
할인액 9,000 (5%)
Each
카트에 추가하기

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This DMEM is modified as follows:
WithWithout
• High Glucose• L-glutamine
• Phenol Red• Sodium Pyruvate
 • HEPES
 • Calcium


The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
사양
세포주HeLa, 293, Cos-7, and PC-12
세포 유형Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형DMEM (Dulbecco's Modified Eagle Medium)
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Liquid
Serum LevelStandard Serum Supplementation
멸균Sterile-filtered
첨가제 포함High Glucose, Phenol Red
첨가제 없음No Glutamine, No HEPES, No Sodium Pyruvate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DMEM, high glucose, no glutamine, no calcium FAQs

인용 및 참조 문헌 (2)

인용 및 참조 문헌
Abstract
Inhibition of adipocyte differentiation by resistin-like molecule alpha. Biochemical characterization of its oligomeric nature.
Authors: Blagoev Blagoy; Kratchmarova Irina; Nielsen Mogens M; Fernandez Minerva M; Voldby Jesper; Andersen Jens S; Kristiansen Karsten; Pandey Akhilesh; Mann Matthias;
Journal:J Biol Chem
PubMed ID:12189153
A novel family of cysteine-rich secreted proteins with unique tissue distribution has recently been identified. One of the members, resistin (for
Oligomerization of G-protein-coupled Receptors Shown by Selective Co-immunoprecipitation.
Authors: Salim Kamran; Fenton Tim; Bacha Jamil; Urien-Rodriguez Hector; Bonnert Tim; Skynner Heather A; Watts Emma; Kerby Julie; Heald Anne; Beer Margaret; McAllister George; Guest Paul C;
Journal:J Biol Chem
PubMed ID:11854302
Recent studies have shown that G-protein-coupled receptors (GPCRs) can assemble as high molecular weight homo- and hetero-oligomeric complexes. This can result in altered receptor-ligand binding, signaling, or intracellular trafficking. We have co-transfected HEK-293 cells with differentially epitope-tagged GPCRs from different subfamilies and determined whether oligomeric complexes were formed by co-immunoprecipitation ... More