Clean-Blot™ IP Detection Kit (HRP)

Thermo Scientific Clean-Blot IP Detection Kit (HRP) contains enough Clean-Blot IP Detection Reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot IP Detection Reagent is a horseradish peroxidase conjugate that is optimized for post-immunoprecipitation Western blot detection of primary antibodies without interference from denatured IP antibody fragments.

The Clean-Blot IP Reagent allows trouble-free Western blot detection of target proteins following IP assays (immunoprecipitation). It works by specifically binding to functional primary antibodies (whole IgG) without also binding to fragments of the IP antibodies, which usually accompany the immunoprecipitated protein through electrophoresis and membrane transfer. The Clean-Blot IP Reagent and Kit eliminate detection-interference from both heavy-chain (approx. 50kDa) and light-chain (25kDa) IgG-fragments of antibodies used for the initial immunoprecipitation assay.

Features of the Clean-Blot IP Detection Reagent:

• Universal—bind and detect most species, IgG subclasses and isotypes of primary antibodies that are commonly used for Western blotting
• Compatible—effective with IP assays performed using Protein A, Protein G, or anti-IgG agarose beads and any blocking buffer
• Cost-effective—eliminates the cost and extra work associated with covalently immobilizing IP antibodies as a means of overcoming Western blot interference
• Flexible—horseradish peroxidase (HRP) reagent for detection with chemiluminescent, fluorescent or colorimetric substrates
• Easy to use—no need to change the Western blotting protocol; simply replace conventional secondary HRP conjugate with the Clean-Blot IP Detection Reagent
• Unobstructed detection—clear Western blot results for immmunoprecipitation assays without significant interference from denatured IgG bands

Included in this kit:
Kit contains Clean-Blot Detection Reagent (HRP), blocking buffer and Thermo Scientific Pierce ECL Substrate (chemiluminescent)

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Clean-Blot™ IP Detection Reagent (HRP)