MagnaBind™ Carboxyl Derivatized Beads

Thermo Scientific™ MagnaBind™ Beads provide a convenient method for magnetic separation of antibodies, antigens, lectins, enzymes, nucleic acids and cells using affinity binding. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

MagnaBind Carboxyl Derivatized Beads are supplied as an aqueous suspension of magnetic iron oxide beads coated with carboxyl groups for covalent coupling of molecules using EDC, a zero-length crosslinker that is amine- and carboxyl-reactive. Additionally, carboxyl derivatized beads have been used to purify plasmid DNA from cells.

Features of MagnaBind Carboxyl Derivatized Beads:

• Composition: Silanized iron oxide
• Magnetization: 25-35EMU/g
• Type of Magnetization: Superparamagnetic (no magnetic memory)
• Surface Area: >100m²/g
• Bead Size: 1-4μm diameter
• Settling Rate: 4% in 30 minutes
• Effective Density: 2.5g/mL
• Number of Beads: 1 x 10⁸ beads/mg
• pH Stability: Aqueous solution, above pH 4.0
• Concentration: ∼20 mg/mL

Applications:

• Cell sorting using either positive or negative selection
• Protein purification or immunoassays using either direct or indirect methods