Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
인용 및 참조 문헌 (5)
Thermo Scientific™

Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format

Thermo Scientific Pierce Sulfo-SMCC는 중간 길이의 cyclohexane spacer arm(8.3 angstrom) 반대편 말단에 NHS-에스테르와 maleimide 반응성 기(group)를 포함하는 수용성 amine-sulfhydryl 교차결합(crosslinker)입니다.Sulfo-SMCC의자세히 알아보기
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카탈로그 번호수량
2232250 mg
A3926810 x 2 mg
221221 g
카탈로그 번호 22322
제품 가격(KRW)
315,000
Online offer
Ends: 31-Dec-2025
350,000
할인액 35,000 (10%)
Each
카트에 추가하기
수량:
50 mg
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
315,000
Online offer
Ends: 31-Dec-2025
350,000
할인액 35,000 (10%)
Each
카트에 추가하기
Thermo Scientific Pierce Sulfo-SMCC는 중간 길이의 cyclohexane spacer arm(8.3 angstrom) 반대편 말단에 NHS-에스테르와 maleimide 반응성 기(group)를 포함하는 수용성 amine-sulfhydryl 교차결합(crosslinker)입니다.

Sulfo-SMCC의 특징:

• 반응성 기(group): sulfo-NHS 에스테르 및 maleimide
• 반응 대상: 아미노 및 sulfhydryl 기(group)
• 수용성(SMCC와 비교)이므로, 생리용액에서 교차결합 가능
• 고순도 결정성 시약을 사용해 고순도 maleimide 활성 유도체를 생성할 수 있음
• 시클로헥산 브릿지(Cyclohexane bridge)는 maleimide 기(group)에 안정성을 더해주므로 Sulfo-SMCC는 단백질의 maleimide 활성에 이상적인 교차결합제임 Maleimide 기(group)는 0.1M sodium phosphate buffer에서 4°C, pH 7의 조건으로 64시간 동안 안정적입니다

Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate(Sulfo-SMCC)는 결합된 부위를 분리하는 것이 불가능하고 막(membrane)을 투과할 수 없는 교차결합(crosslinker)입니다. 또한, 아민 반응성 N-hydroxysuccinimide(NHS 에스테르)와 sulfhydryl 반응성 maleimide 기(group)를 함유합니다. NHS 에스테르는 pH 7-9에서 일차 아민과 반응하여 안정적인 amide 결합을 형성합니다. Maleimide는 pH 6.5-7.5에서 sulfhydryl 기(group)와 반응하여 안정적인 thioether 결합을 형성합니다. Sulfo-SMCC의 maleimide 기(group)와 SMCC는 spacer arm의 시클로헥산 브릿지(Cyclohexane bridge) 덕분에 최대 pH 7.5까지 매우 안정적입니다. Sulfo-SMCC는 친수성 sulfonyl moiety를 함유하고 있어 물과 일반적으로 사용되는 많은 버퍼에서 최대 약 100mM까지 용해되므로, 단백질 구조를 동요시킬 수 있는 유기 용매를 사용하지 않아도 됩니다.

어플리케이션:
• 항체의 효소 표지 - 효소와 항체 특이성을 유지할 수 있음
• 1단계 또는 2단계 교차결합 반응을 통해 특이적 생체접합체 생성
• hapten 연결을 위한 sulfhydryl 반응성 maleimide 활성 캐리어 단백질 생성

사양:
Sulfo-SMCC는 최고의 사양에 따라 제조되며 가장 특이적인 생체접합체와 maleimide 활성 단백질을 생성하므로 데이터 무결성이 보장되고 최고의 교차결합 일관성을 나타냅니다. 각 Sulfo-SMCC 로트는 시험을 거쳐 다음 최소 사양을 충족합니다.

• 동일성: IR 스캔은 Sulfo-SMCC 교차결합(crosslinker)의 구조와 작용기(functional group) 특유의 피크만을 나타냄
• 순도: ≥ 정량적 NMR에서 90%(교차결합[crosslinker] 순도에서 가장 높은 표준)
• 용해도: ≥ DI 물에서 10mg/mL

제품 참고문헌:
교차결합(Crosslinker) 어플리케이션 가이드—이 제품에 대한 최신 참조 문헌 검색

관련 자료:
교차결합 개요
교차결합의 화학

관련 제품:
Crosslinkers at a glance(선택 가이드)
SMCC
Bioconjugate Techniques(도서)
Maleimide Activated Carrier Proteins and Kits
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 투과성No
설명Sulfo-SMCC
형태Powder
라벨링 방법Chemical Labeling
분자량436.37
페길레이티드No
제품라인Pierce
수량50 mg
반응성 부분Maleimide, Sulfo-NHS Ester
배송 조건Ambient
용해도Water
스페이서 암 길이8.3 Å
수용성Yes
화학물질 반응성Amine-Sulfhydryl
CleavableNo
교차결합제 유형Heterobifunctional
형식Standard, Single-use, Premium-grade
제품 유형Crosslinker
스페이서Short (<10 Å)
Unit SizeEach
구성 및 보관
Upon receipt store desiccated at -20°C.

자주 묻는 질문(FAQ)

How long is Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) (Cat. No. 22322) functional group (maleimide) stable for after the amine targeted end is occupied?

The Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) (Cat. No. 22322) maleimide group is stable for 64 hours in 0.1 M sodium buffer, pH 7 at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can you provide the shelf-life for Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)?

Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the shelf life of Sulfo-SMCC (Cat. No. 22322)?

Sulfo-SMCC is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (desiccated at -20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the difference between SMCC and Sulfo-SMCC?

SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). Sulfo-SMCC is a similar compound but it has a sulfonate group on the NHS ring which makes it water-soluble whereas SMCC is water-insoluble and needs to be dissolved first in an organic solvent like DMF or DMSO. Subsequent dilution into aqueous reaction buffer is generally possible, and most protein reactants will remain soluble if the final concentration of organic solvent is less than 10%.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is the Thermo Scientific IP Lysis Buffer similar to RIPA buffer?

The Thermo Scientific IP Lysis Buffer (Cat. No. 87787) is a mammalian whole cell lysis buffer based on a modified RIPA buffer formulation without SDS. This moderate strength lysis buffer is able to effectively solubilize cellular proteins but does not liberate genomic DNA or disrupt protein complexes like RIPA buffer.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), 50 mg FAQs

인용 및 참조 문헌 (5)

인용 및 참조 문헌
Abstract
Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms.
Authors:Adessi C, Matton G, Ayala G, Turcatti G, Mermod JJ, Mayer P, Kawashima E
Journal:Nucleic Acids Res
PubMed ID:11024189
Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides ... More
Substrate-induced conformational change in bacterial complex I.
Authors:Mamedova AA, Holt PJ, Carroll J, Sazanov LA
Journal:J Biol Chem
PubMed ID:15037611
'The mechanism coupling electron transfer and proton pumping in respiratory complex I (NADH-ubiquinone oxidoreductase) has not been established, but it has been suggested that it involves conformational changes. Here, the influence of substrates on the conformation of purified complex I from Escherichia coli was studied by cross-linking and electron microscopy. ... More
Chondrex: new marker of joint disease.
Authors:Harvey S, Weisman M, O'Dell J, Scott T, Krusemeier M, Visor J, Swindlehurst C
Journal:Clin Chem
PubMed ID:9510855
Chondrex, a major secretory protein of human chondrocytes and synovial fibroblasts, is increased in serum of patients with joint and cartilage disease. We have developed a sandwich-type ELISA for quantifying chondrex in serum. The interassay CVs were 2.8-3.7% and the average within-run and total CVs were 3.6% and 5.4%, respectively. ... More
Development of sensitive immunoassays to detect amylin and amylin-like peptides in unextracted plasma.
Authors:Percy AJ, Trainor DA, Rittenhouse J, Phelps J, Koda JE
Journal:Clin Chem
PubMed ID:8605675
Amylin is a 37-amino-acid polypeptide synthesized in and secreted from pancreatic beta cells along with insulin. Its biological actions include the slowing and reduction of postmeal increases in plasma glucose concentrations. Studies of the basic amylin biology in humans have been hampered by the lack of a rapid, sensitive assay ... More
Sulfhydryl-based tumor antigen-carrier protein conjugates stimulate superior antitumor immunity against B cell lymphomas.
Authors:Betting DJ, Kafi K, Abdollahi-Fard A, Hurvitz SA, Timmerman JM
Journal:J Immunol
PubMed ID:18768870
Therapeutic vaccination of B cell lymphoma patients with tumor-specific Ig (idiotype, or Id) chemically coupled to the immunogenic foreign carrier protein keyhole limpet hemocyanin (KLH) using glutaraldehyde has shown promising results in early clinical trials, and phase III trials are underway. However, glutaraldehyde Id-KLH vaccines fail to elicit anti-Id immune ... More