RPMI 1640 Medium, HEPES
RPMI 1640 Medium, HEPES
인용 및 참조 문헌 (7)
Gibco™

RPMI 1640 Medium, HEPES

RPMI 1640 Medium은 원래 인간 백혈병 세포의 단일 세포층(monolayer) 혹은 현탁(suspension) 배양을 위해 개발된 제품입니다. Roswell Park Memorial Institute (RPMI)자세히 알아보기
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카탈로그 번호수량
22400089500mL
22400097100 mL
2240010510 x 500mL
224000711000mL
224001216 x 1000mL
카탈로그 번호 22400089
제품 가격(KRW)
65,000
Online offer
Ends: 31-Dec-2025
68,000
할인액 3,000 (4%)
Each
카트에 추가하기
수량:
500mL
Customize this product
제품 가격(KRW)
65,000
Online offer
Ends: 31-Dec-2025
68,000
할인액 3,000 (4%)
Each
카트에 추가하기
RPMI 1640 Medium은 원래 인간 백혈병 세포의 단일 세포층(monolayer) 혹은 현탁(suspension) 배양을 위해 개발된 제품입니다. Roswell Park Memorial Institute (RPMI) 1640 Medium은 HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas 등의 다양한 포유류 세포에 적합한 것으로 확인되었습니다. 당사는 광범위한 세포 배양 응용 분야에 따라 다양하게 조성이 변경된 RPMI 1640 Medium를 제공합니다. 배지 선택 도구를 사용하여 적절한 조성을 간편하게 찾을 수 있습니다.

이 RPMI은 다음과 같이 조성이 변경되었습니다.
포함제외 성분
• L-글루타민
• HEPES
• 페놀 레드

모든 조성을 이용할 수 있습니다.

RPMI의 사용
RPMI1640 배지는 다른 배지에 비해 특징적으로 환원제 글루타티온과 고농도의 비타민을 함유하고 있습니다. RPMI 1640 medium에는 Eagle's Minimal Essential Medium이나 Dulbecco's Modified Eagle Medium에는 없는 biotin, vitamin B12, PABA가 포함되어 있습니다. 또한 비타민 이노시톨과 콜린도 매우 높은 농도로 함유되어 있습니다. RPMI 1640 Medium에는 단백질, 지질, 성장인자가 들어있지 않습니다. 따라서 RPMI 1640 배지는 일반적으로 10% 태아 소 혈청(FBS)을 보충해야 합니다. RPMI 1640 배지는 중탄산나트륨(sodium bicarbonate) 버퍼 시스템(2.0 g/L)을 이용하며, 이로 인해 생리학적인 pH를 유지하기 위해 5–10% CO2 환경이 요구됩니다.

cGMP 제조 및 품질 시스템
RPMI 1640 배지는 뉴욕주의 Grand Island에 위치한 cGMP 준수 시설에서 제조됩니다. 이 시설은 의료기기 제조원으로 FDA에 등록되어 있으며 ISO 13485 표준 인증을 받은 기관입니다. 공급망 유지를 위해 당사는 당사의 스코틀랜드 시설에서도 동일한 RPMI 1640 제품을 제공합니다(52400-025). 이 시설은 의료기기 제조원으로 FDA에 등록되어 있으며 ISO 13485 표준 인증을 받은 기관입니다.
제품 용도: 이 제품은 체외 진단용입니다. 주의: 사람이나 동물의 치료용으로 사용할 수 없습니다. 해당 용도 외 다른 용도로 사용 시 현지 관련 법에 위배될 수 있습니다.
사양
세포주HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes 및 carcinomas
세포 유형백혈병 세포
농도1X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
수량500mL
유통 기한제조일로부터 12개월
배송 조건실온
분류동물 원료 성분 비함유
형태액체
Serum LevelStandard Serum Supplementation
멸균멸균 여과
Sterilization MethodSterile-filtered
첨가제 포함L-글루타민, HEPES, 페놀 레드, HEPES, Phenol Red
첨가제 없음No Sodium Pyruvate
Unit SizeEach
구성 및 보관
보관 조건: 2∼8°C. 차광 보관
배송 조건: 상온
유통 기한: 제조일로부터 12개월

자주 묻는 질문(FAQ)

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?

We do not provide stability data for the product once it is opened as it would depend on the usage and storage conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all RPMI 1640 Medium, HEPES FAQs

인용 및 참조 문헌 (7)

인용 및 참조 문헌
Abstract
A functional role for the B56 alpha-subunit of protein phosphatase 2A in ceramide-mediated regulation of Bcl2 phosphorylation status and function.
Authors: Ruvolo Peter P; Clark Warren; Mumby Marc; Gao Fengqin; May W Stratford;
Journal:J Biol Chem
PubMed ID:11929874
'Recently it has been shown that the potent apoptotic agent ceramide activates a mitochondrial protein phosphatase 2A (PP2A) and promotes dephosphorylation of the anti-apoptotic molecule Bcl2 (Ruvolo, P. P., Deng, X., Ito, T., Carr, B. K., and May, W. S. (1999) J. Biol. Chem. 274, 20296-20300). In cells expressing Bcl2, ... More
Cell-penetrating Peptide enhanced intracellular Raman imaging and photodynamic therapy.
Authors:Fales AM, Yuan H, Vo-Dinh T
Journal:
PubMed ID:23659475
'We present the application of a theranostic system combining Raman imaging and the photodynamic therapy (PDT) effect. The theranostic nanoplatform was created by loading the photosensitizer, protoporphyrin IX, onto a Raman-labeled gold nanostar. A cell-penetrating peptide, TAT, enhanced intracellular accumulation of the nanoparticles in order to improve their delivery and ... More
Slow-cycling therapy-resistant cancer cells.
Authors:Moore N, Houghton J, Lyle S
Journal:Stem Cells Dev
PubMed ID:21973238
Tumor recurrence after chemotherapy is a major cause of patient morbidity and mortality. Recurrences are thought to be secondary to small subsets of cancer cells that are better able to survive traditional forms of chemotherapy and thus drive tumor regrowth. The ability to isolate and better characterize these therapy-resistant cells ... More
Krüppel-like zinc fingers bind to nuclear import proteins and are required for efficient nuclear localization of erythroid Krüppel-like factor.
Authors: Quadrini Karen J; Bieker James J;
Journal:J Biol Chem
PubMed ID:12072445
Erythroid Krüppel-like Factor (EKLF/KLF-1) is an erythroid-specific transcription factor that contains three C(2)H(2) zinc fingers and is required for correct chromatin structure and expression of the beta-globin locus. However, regions within the EKLF protein that serve as signals for its nuclear localization and the proteins that may enable it to ... More
Natural truncation of the chemokine MIP-1 beta /CCL4 affects receptor specificity but not anti-HIV-1 activity.
Authors: Guan Ennan; Wang Jinhai; Roderiquez Gregory; Norcross Michael A;
Journal:J Biol Chem
PubMed ID:12070155
Activated lymphocytes synthesize and secrete substantial amounts of the beta-chemokines macrophage inflammatory protein (MIP)-1 alpha/CCL3 and MIP-1 beta/CCL4, both of which inhibit infection of cells with human immunodeficiency virus type 1 (HIV-1). The native form of MIP-1 beta secreted by activated human peripheral blood lymphocytes (MIP-1 beta(3-69)) lacks the two ... More
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