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인용 및 참조 문헌 (1)
Thermo Scientific™
Pierce™ Reversible Protein Stain Kit for Nitrocellulose Membranes
The Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes is a rapid and sensitive alternative to Ponceau S stain for자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 24580 | 1.5 L |
카탈로그 번호 24580
제품 가격(KRW)
238,000
キャンペーン価格
Ends: 31-Dec-2025
280,000할인액 42,000 (15%)
Each
수량:
1.5 L
제품 가격(KRW)
238,000
キャンペーン価格
Ends: 31-Dec-2025
280,000할인액 42,000 (15%)
Each
The Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on nitrocellulose membranes after transfer from polyacrylamide gels.
Features:
• Sensitive—high-avidity, total-protein stain; lower limit of detection equals 25 to 50 ng per band
• Specific—detects only protein; does not bind or interact with other electrophoresis or sample components
• Protocol—stain in less than five minutes; completely erase and reverse staining in less than 15 minutes
• Stability—components are stable at room temperature, saving refrigerator space and eliminating equilibration steps
Compare all membrane stains ›
This kit for membrane staining uses a nondestructive, reversible, reliable, and sensitive method to stain and detect proteins on nitrocellulose membranes. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents. The staining protocol is simple and quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane.
Features:
• Sensitive—high-avidity, total-protein stain; lower limit of detection equals 25 to 50 ng per band
• Specific—detects only protein; does not bind or interact with other electrophoresis or sample components
• Protocol—stain in less than five minutes; completely erase and reverse staining in less than 15 minutes
• Stability—components are stable at room temperature, saving refrigerator space and eliminating equilibration steps
Compare all membrane stains ›
This kit for membrane staining uses a nondestructive, reversible, reliable, and sensitive method to stain and detect proteins on nitrocellulose membranes. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents. The staining protocol is simple and quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane.
For Research Use Only. Not for use in diagnostic procedures.
사양
설명Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes
검출 위치In-Blot Detection
검출 방법Colorimetric
제품라인Pierce
제품 유형Reversible Protein Stain Kit
수량1.5 L
타겟 분자Protein
라벨 또는 염료Proprietary Mix
Unit SizeEach
구성 및 보관
Sufficient For: 10 nitrocellulose membranes (8 cm x 8 cm) from mini gels (SDS-PAGE)
• Reversible Stain, 250 mL
• Destain, 2 x 500 mL
• Stain Eraser, 250 mL
Store at room temperature
• Reversible Stain, 250 mL
• Destain, 2 x 500 mL
• Stain Eraser, 250 mL
Store at room temperature
자주 묻는 질문(FAQ)
How can I increase the signal intensity when I am using SuperSignal West Femto Maximum Sensitivity Substrate?
How can I increase the signal intensity when I am using SuperSignal West Dura Extended Duration Substrate?
인용 및 참조 문헌 (1)
인용 및 참조 문헌
Abstract
A high-affinity reversible protein stain for Western blots.
Journal:Anal Biochem
PubMed ID:15158487
'We describe a reversible staining technique, using MemCode, a reversible protein stain by which proteins can be visualized on nitrocellulose and polyvinylidine fluoride (PVDF) membranes without being permanently fixed to the membrane itself. This allows subsequent immunoblot analysis of the proteins to be performed. The procedure is applicable only to