L-Glutamine (200 mM)
L-Glutamine (200 mM)
Gibco™

L-Glutamine (200 mM)

L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidine자세히 알아보기
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카탈로그 번호수량
25030081100 mL
2503014920 mL
2503016420 x 100 mL
카탈로그 번호 25030081
제품 가격(KRW)
59,000
Online offer
Ends: 31-Dec-2025
62,000
할인액 3,000 (5%)
Each
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수량:
100 mL
Customize this product
제품 가격(KRW)
59,000
Online offer
Ends: 31-Dec-2025
62,000
할인액 3,000 (5%)
Each
카트에 추가하기
L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. Unlike most other amino acids, L-glutamine is not stable in solution. The rate at which degradation proceeds is a function of time, temperature, and pH. Gibco™ L-glutamine is a ready-to-use 200 mM stock solution that acts as a cell culture supplement. The optimal concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2–6 mM.

We also offer Gibco™ GlutaMAX™ Supplement as a stable alternative to L-glutamine.

Dual-site cGMP Manufacturing and Quality System
Gibco™ L-glutamine is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ L-glutamine product made in our Grand Island facility (25030-081). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

This product is not used for in vitro diagnostic purpose in some countries.
사양
화학물질 이름 또는 재질Glutamine
Concentration or Composition (by Analyte or Components)100X
그린 기능Sustainable packaging
물리적 형태Liquid
제품라인Gibco
권장 스토리지Storage conditions: -5°C to -20°C. Protect from light.
Shipping conditions: Dry ice
Shelf life: 24 months from date of manufacture
유통 기한24 Months
배송 조건Dry Ice
수량100 mL
pH5 to 6
Unit SizeEach

자주 묻는 질문(FAQ)

Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution have “Product of Brazil” on the label?

L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution is made in the USA from a raw material glutamine powder manufactured in Brazil. The country of manufacture of a component that has been subjected to manufacturing or processing operations in more than one country is the last country in which the component has been “substantially transformed”. A “substantial transformation” occurs in a country when a product emerges from a manufacturing process with a name, character, or use different from that of the original material(s) subjected to that process.

Diluting, purifying, sorting, testing, packing, repacking, or affixing labels to a product in a country does not constitute a “substantial transformation” in that country, hence the label must bear the country of the original raw material origin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My order of L-Glutamine (Cat. No. 25030081) arrived frozen. Is it stable?

If L-Glutamine is completely or partially frozen, it is still stable. It becomes unstable when stored for extended periods of time completely thawed, especially above 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is your recommended method for thawing L-Glutamine before adding to cell culture media?

We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why did my 200 mM L-glutamine precipitate when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37 degrees C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

인용 및 참조 문헌 (10)

인용 및 참조 문헌
Abstract
Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C.
Authors:Friedrich U, Blom AM, Dahlbäck B, Villoutreix BO,
Journal:J Biol Chem
PubMed ID:11316800
'Human activated protein C (APC) is a key component of a natural anticoagulant system that regulates blood coagulation. In vivo, the catalytic activity of APC is regulated by two serpins, alpha1-antitrypsin and the protein C inhibitor (PCI), the inhibition by the latter being stimulated by heparin. We have identified a ... More
FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells.
Authors:Börner S, Schwede F, Schlipp A, Berisha F, Calebiro D, Lohse MJ, Nikolaev VO,
Journal:Nat Protoc
PubMed ID:21412271
'Real-time measurements of second messengers in living cells, such as cAMP, are usually performed by ratiometric fluorescence resonance energy transfer (FRET) imaging. However, correct calibration of FRET ratios, accurate calculations of absolute cAMP levels and actual permeabilities of different cAMP analogs have been challenging. Here we present a protocol that ... More
A critical role for choline kinase-alpha in the aggressiveness of bladder carcinomas.
Authors:Hernando E, Sarmentero-Estrada J, Koppie T, Belda-Iniesta C, Ramírez de Molina V, Cejas P, Ozu C, Le C, Sánchez JJ, González-Barón M, Koutcher J, Cordón-Cardó C, Bochner BH, Lacal JC, Ramírez de Molina A,
Journal:Oncogene
PubMed ID:19448670
'Bladder cancer is one of the most common causes of death in industrialized countries. New tumor markers and therapeutic approaches are still needed to improve the management of bladder cancer patients. Choline kinase-alpha (ChoKalpha) is a metabolic enzyme that has a role in cell proliferation and transformation. Inhibitors of ChoKalpha ... More
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.
Authors:Walev I, Bhakdi SC, Hofmann F, Djonder N, Valeva A, Aktories K, Bhakdi S,
Journal:Proc Natl Acad Sci U S A
PubMed ID:11248053
'The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)-10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca(2+)-calmodulin and on ... More
Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.
Authors:Nandivada H, Villa-Diaz LG, O'Shea KS, Smith GD, Krebsbach PH, Lahann J,
Journal:Nat Protoc
PubMed ID:21720316
The culture of human embryonic stem (hES) cells in defined and xenogeneic-free conditions will contribute substantially to future biotechnological and medical applications. To achieve this goal, we developed the first fully defined synthetic polymer coating poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) that sustains long-term growth of hES cells in different culture media. ... More