L-Glutamine (200 mM)
L-Glutamine (200 mM)
Gibco™

L-Glutamine (200 mM)

L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidine자세히 알아보기
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카탈로그 번호수량
2503014920 mL
25030081100 mL
2503016420 x 100 mL
카탈로그 번호 25030149
제품 가격(KRW)
26,000
Online offer
Ends: 31-Dec-2025
27,000
할인액 1,000 (4%)
Each
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수량:
20 mL
Customize this product
제품 가격(KRW)
26,000
Online offer
Ends: 31-Dec-2025
27,000
할인액 1,000 (4%)
Each
카트에 추가하기
L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. Unlike most other amino acids, L-glutamine is not stable in solution. The rate at which degradation proceeds is a function of time, temperature, and pH. Gibco™ L-glutamine is a ready-to-use 200 mM stock solution that acts as a cell culture supplement. The optimal concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2–6 mM.

We also offer Gibco™ GlutaMAX™ Supplement as a stable alternative to L-glutamine.

Dual-site cGMP Manufacturing and Quality System
Gibco™ L-glutamine is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ L-glutamine product made in our Grand Island facility (25030-081). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

This product is not used for in vitro diagnostic purpose in some countries.
사양
화학물질 이름 또는 재질Glutamine
Concentration or Composition (by Analyte or Components)100X
물리적 형태Liquid
권장 스토리지Storage conditions: -5 to -20°C. Protect from light.
Shipping conditions: Frozen
Shelf life: 24 months from date of manufacture
유통 기한24 Months
배송 조건Dry Ice
수량20 mL
pH5 to 6
Unit SizeEach

자주 묻는 질문(FAQ)

Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My order of L-Glutamine (Cat. No. 25030081) arrived frozen. Is it stable?

If L-Glutamine is completely or partially frozen, it is still stable. It becomes unstable when stored for extended periods of time completely thawed, especially above 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is your recommended method for thawing L-Glutamine before adding to cell culture media?

We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why did my 200 mM L-glutamine precipitate when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37 degrees C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

인용 및 참조 문헌 (14)

인용 및 참조 문헌
Abstract
Metabolism of 4 beta -hydroxycholesterol in humans.
Authors: Bodin Karl; Andersson Ulla; Rystedt Eva; Ellis Ewa; Norlin Maria; Pikuleva Irina; Eggertsen Gösta; Björkhem Ingemar; Diczfalusy Ulf;
Journal:J Biol Chem
PubMed ID:12077124
'One of the major oxysterols in the human circulation is 4 beta-hydroxycholesterol formed from cholesterol by the drug-metabolizing enzyme cytochrome P450 3A4. Deuterium-labeled 4 beta-hydroxycholesterol was injected into two healthy volunteers, and the apparent half-life was found to be 64 and 60 h, respectively. We have determined earlier the half-lives ... More
Activation of retinoic acid receptor-dependent transcription by all-trans-retinoic acid metabolites and isomers.
Authors: Idres Nadia; Marill Julie; Flexor Maria A; Chabot Guy G;
Journal:J Biol Chem
PubMed ID:12070176
'We have shown that four metabolites of all-trans-retinoic acid (ATRA) (4-oxo-, 4-OH-, 18-OH-, and 5,6-epoxy-RA) can induce maturation of NB4 promyelocytic leukemia cells (Idres, N., Benoit, G., Flexor, M. A., Lanotte, M., and Chabot, G. G. (2001) Cancer Res. 61, 700-705). To better understand the mechanism of action of ATRA ... More
Inhibition of transforming growth factor beta signaling and Smad-dependent activation of transcription by the Latent Membrane Protein 1 of Epstein-Barr virus.
Authors: Prokova Vassiliki; Mosialos George; Kardassis Dimitris;
Journal:J Biol Chem
PubMed ID:11781310
'Inhibition of transforming growth factor beta (TGFbeta) signaling by the Epstein-Barr virus Latent Membrane Protein 1 (LMP1) may account, at least in part, for the oncogenic activity of LMP1. We found that LMP1 is a potent inhibitor of TGFbeta signaling and Smad-dependent activation of transcription in 293 epithelial cells and ... More
Interaction codes within the family of mammalian Phox and Bem1p domain-containing proteins.
Authors:Lamark T, Perander M, Outzen H, Kristiansen K, Øvervatn A, Michaelsen E, Bjørkøy G, Johansen T,
Journal:J Biol Chem
PubMed ID:12813044
'The Phox and Bem1p (PB1) domain constitutes a recently recognized protein-protein interaction domain found in the atypical protein kinase C (aPKC) isoenzymes, lambda/iota- and zeta PKC; members of mitogen-activated protein kinase (MAPK) modules like MEK5, MEKK2, and MEKK3; and in several scaffold proteins involved in cellular signaling. Among the last ... More
Molecular rearrangements of the extracellular vestibule in NMDAR channels during gating.
Authors: Sobolevsky Alexander I; Beck Christine; Wollmuth Lonnie P;
Journal:Neuron
PubMed ID:11779481
Many N-methyl-D-aspartate receptor (NMDAR) channel blockers that have therapeutic potential can be trapped in the closed state. Using a combination of the substituted cysteine accessibility method and open channel blockers, we found that the M3 segment forms the core of the extracellular vestibule, including a deep site for trapping blockers. ... More