Hypersil GOLD™ Peptide HPLC Column
Thermo Scientific™

Hypersil GOLD™ Peptide HPLC Column

Hypersil GOLD™ Peptide performance is assured by our dual quality confirmation, which is performed on both neutrals compounds as well as on a Cytochrome C digest.
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카탈로그 번호길이(미터법)최대 압력
26002-102130100 mm21756 psi (1500 bar)
26002-05213050 mm14503 psi (1000 bar)
26002-152130150 mm21756 psi (1500 bar)
카탈로그 번호 26002-102130
제품 가격(KRW)
1,943,000
Each
길이(미터법):
100 mm
최대 압력:
21756 psi (1500 bar)
제품 가격(KRW)
1,943,000
Each

Resolve peptide challenges with one column - NEW Hypersil GOLD Peptide.

Achieve consistent retention of all peptides, including hydrophilic ones, and resolve deamidated species. Designed for stability and lot-to-lot consistency, this column meets the needs of biopharmaceutical product development and manufacturing control.

Hypersil GOLD Peptide column performance is assured through dual quality confirmation, including a peptide-based QC test, ensures confidence in ultrapure silica, ligand bonding, and column packing for unparalleled sequence coverage and higher peak capacity compared to other peptide columns.

Consistent, Reliable Results

Enjoy excellent reproducibility with minimal RSD%, ensuring consistency from column-to-column and lot-to-lot.


Optimized Peptide Retention - Retain More, Discover More

Our column uniquely holds onto hydrophilic peptides, ensuring no critical data is missed.


Exceptional Peak Capacity - Separate with Superiority

Achieve higher peak capacity for detailed and clear separations, ensuring every component is distinctly resolved.


Advanced PTM Analysis - Decipher Complex Modifications

Efficiently separate key post-translational modifications like deamidation, crucial for in-depth protein analysis. Excellent peak shape for peptides using either FA- or TFA-containing mobile phases.


Unparalleled Sequence Coverage - See the Full Picture

High sequence coverage provides a comprehensive analysis, revealing the true complexity of your samples.

사양
컬럼 유형Reversed Phase
엔드캡 처리됨Yes
용도LC/MS
용도(애플리케이션)Peptide
최대 압력21756 psi (1500 bar)
팩킹 물질Spherical, Fully Porous, Ultrapure Silica
입자 형상1.9 μm
수량1 Each
표면적220 m²/g
온도60°C
USP 유형L1
탄소 부하11%
직경(미터법)2.1 mm
길이(미터법)100 mm
물질Silica
Reversed Phase
기공 크기175 Å
제품라인Hypersil GOLD
기술Reversed Phase HPLC
유형Analytical Column
pH2 to 11
Unit SizeEach

자주 묻는 질문(FAQ)

What application areas or workflows are best served by Hypersil GOLD Peptide columns?

Hypersil GOLD Peptide columns are particularly suited for:
• Biopharmaceutical peptide mapping and manufacturing control (where PTMs, deamidation, and hydrophilic peptides matter).
• LC-MS peptide workflows include digest analysis, proteomics, and glycopeptide variant monitoring.

What mobile phase or buffer considerations should I be aware of when using Hypersil GOLD Peptide columns?

You need to ensure that your sample is fully dissolved and free of particles (e.g., filter 0.2 µm or centrifuge) before injection. The column supports both FA- and TFA-containing mobile phases with excellent peak shape for peptides. For column equilibration, you need to flush new columns with at least 10 column volumes of the new mobile phase. Additionally, take into consideration that at higher pH (>8) and elevated temperature, column lifetime may be shortened.

How do Hypersil GOLD Peptide columns handle hydrophilic peptides, which often elute too early on traditional columns?

Hypersil GOLD Peptide columns are unique in that they hold onto hydrophilic peptides, ensuring no critical data is missed. In other words, it has been engineered (via the packing material, ligand density and pore structure) to provide better retention of less-hydrophobic peptide species compared to generic RP columns, to reduce early elution or loss of critical peptides in a digest map.

What makes Hypersil GOLD Peptide columns specifically suited for peptide analysis?

The Hypersil GOLD Peptide column is built on 1.9 µm, 175 Å pore size spherical, fully porous ultrapure silica, specifically optimized for peptide separations. It provides consistent retention of all peptides—including hydrophilic peptides—and resolves post-translational modifications such as deamidation. Compared with many general-purpose C18 peptide columns, it offers higher peak capacity, better retention of hydrophilic peptides, and enhanced sequence coverage.