Fetal Bovine Serum, qualified, 미국
Fetal Bovine Serum, qualified, 미국
인용 및 참조 문헌 (33)
Gibco™

Fetal Bovine Serum, qualified, 미국

Gibco fetal bovine sera는 기본 세포 배양, 특수 연구 및 특정 분석에 뛰어난 가치를 제공하며, 연구자의 연구 요구 사항과 예산자세히 알아보기
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카탈로그 번호수량형식
26140079500 mL병
Premium Fetal Bovine Serum (FBS) 은 안정성 및 제조 공정은 그대로 유지되면서 품질이 더욱 강화되었습니다.
카탈로그 번호 26140079
현재 제품 선택
제품 가격(KRW)
768,000
Each
카트에 추가하기
Gibco fetal bovine sera는 기본 세포 배양, 특수 연구 및 특정 분석에 뛰어난 가치를 제공하며, 연구자의 연구 요구 사항과 예산 요구 사항을 충족할 수 있도록 일관된 품질 및 우수성을 널리 인정받은 지원을 통해 신뢰할 수 있는 결과를 제공합니다.

Gibco 혈청: 일관된 성능을 위한 믿을 수 있는 품질

Gibco 혈청 카테고리: Value Plus(Performance)
• 일반적인 세포주를 사용한 일반 세포 배양 용도
• 낮은 엔도톡신 및 헤모글로빈 함량
• 엔도톡신 함량: < 10 EU/mL
• 헤모글로빈 함량: < 25 mg/dL
• 원산지: 미국

Gibco 혈청이 제공하는 이점
• ISO 13485 인증, FDA 등록 시설에서 처리
• 체외 진단용 라벨(최고 수준의 USP 멸균 시험 준수)
• 0.1 μm에서 삼중 필터링됨
• Gibco 병은 후드 안에서도 사용이 간편하고 오염 위험을 줄이며 세포 배양을 보다 일관성 있게 수행할 수 있도록 도와줍니다.
• 떼어내기 쉬운 라벨 스티커는 실험실 노트북에 붙여서 로트 번호 및 유효기간 기록을 쉽게 참조할 수 있도록 해줍니다.
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
사양
엔도톡신 농도≤ 10 EU/mL
헤모글로빈 농도≤ 25 mg⁄dl
순도 또는 품질 등급적합
배송 조건냉동 상태
종소
Age태아
원산지미국
형태액체
형식병
제품 유형소 태아 혈청(Fetal Bovine Serum)
수량500 mL
Serum TreatmentStandard (Sterile-Filtered)
멸균멸균
Sterilization MethodTriple-filtered, 0.1 μm
Unit SizeEach
구성 및 보관
보관 조건: ≤-10°C
배송 조건: 냉동 상태

자주 묻는 질문(FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, qualified, 미국 FAQs

인용 및 참조 문헌 (33)

인용 및 참조 문헌
Abstract
A functional role for the B56 alpha-subunit of protein phosphatase 2A in ceramide-mediated regulation of Bcl2 phosphorylation status and function.
Authors: Ruvolo Peter P; Clark Warren; Mumby Marc; Gao Fengqin; May W Stratford;
Journal:J Biol Chem
PubMed ID:11929874
'Recently it has been shown that the potent apoptotic agent ceramide activates a mitochondrial protein phosphatase 2A (PP2A) and promotes dephosphorylation of the anti-apoptotic molecule Bcl2 (Ruvolo, P. P., Deng, X., Ito, T., Carr, B. K., and May, W. S. (1999) J. Biol. Chem. 274, 20296-20300). In cells expressing Bcl2, ... More
Metabolism of 4 beta -hydroxycholesterol in humans.
Authors: Bodin Karl; Andersson Ulla; Rystedt Eva; Ellis Ewa; Norlin Maria; Pikuleva Irina; Eggertsen Gösta; Björkhem Ingemar; Diczfalusy Ulf;
Journal:J Biol Chem
PubMed ID:12077124
'One of the major oxysterols in the human circulation is 4 beta-hydroxycholesterol formed from cholesterol by the drug-metabolizing enzyme cytochrome P450 3A4. Deuterium-labeled 4 beta-hydroxycholesterol was injected into two healthy volunteers, and the apparent half-life was found to be 64 and 60 h, respectively. We have determined earlier the half-lives ... More
Activation of retinoic acid receptor-dependent transcription by all-trans-retinoic acid metabolites and isomers.
Authors: Idres Nadia; Marill Julie; Flexor Maria A; Chabot Guy G;
Journal:J Biol Chem
PubMed ID:12070176
'We have shown that four metabolites of all-trans-retinoic acid (ATRA) (4-oxo-, 4-OH-, 18-OH-, and 5,6-epoxy-RA) can induce maturation of NB4 promyelocytic leukemia cells (Idres, N., Benoit, G., Flexor, M. A., Lanotte, M., and Chabot, G. G. (2001) Cancer Res. 61, 700-705). To better understand the mechanism of action of ATRA ... More
Interaction of human breast fibroblasts with collagen I increases secretion of procathepsin B.
Authors: Koblinski Jennifer E; Dosescu Julie; Sameni Mansoureh; Moin Kamiar; Clark Katherine; Sloane Bonnie F;
Journal:J Biol Chem
PubMed ID:12072442
'Interactions of stromal and tumor cells with the extracellular matrix may regulate expression of proteases including the lysosomal proteases cathepsins B and D. In the present study, we determined whether the expression of these two proteases in human breast fibroblasts was modulated by interactions with the extracellular matrix component, collagen ... More
A ligand-inducible epidermal growth factor receptor/anaplastic lymphoma kinase chimera promotes mitogenesis and transforming properties in 3T3 cells.
Authors: Piccinini Gina; Bacchiocchi Roberta; Serresi Michela; Vivani Caterina; Rossetti Silvia; Gennaretti Claudia; Carbonari Damiano; Fazioli Francesca;
Journal:J Biol Chem
PubMed ID:11919185
'Oncogenic rearrangements of the anaplastic lymphoma kinase (ALK) gene, encoding a receptor type tyrosine kinase, are frequently associated with anaplastic large cell lymphomas. Such rearrangements juxtapose the intracellular domain of ALK to 5''-end sequences belonging to different genes and create transforming fusion proteins. To understand how the oncogenic versions of ... More
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