PageRuler™ Prestained Protein Ladder, 10 to 180 kDa
PageRuler™ Prestained Protein Ladder, 10 to 180 kDa
Thermo Scientific™

PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

Thermo Scientific PageRuler Prestained Protein Ladder는 청색, 주황색, 녹색으로 염색된 단백질(10-180kDa) 10개로 구성된 혼합물로서 단백질 전기영동(SDS-PAGE)과 웨스턴 블로팅(Western blotting)에서 크기자세히 알아보기
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호수량
266162 x 250 μL
26616X48 x 250 μL
2661710 x 250 μL
카탈로그 번호 26616
제품 가격(KRW)
212,000
キャンペーン価格
Ends: 31-Dec-2025
235,000
할인액 23,000 (10%)
Each
카트에 추가하기
수량:
2 x 250 μL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
212,000
キャンペーン価格
Ends: 31-Dec-2025
235,000
할인액 23,000 (10%)
Each
카트에 추가하기
Thermo Scientific PageRuler Prestained Protein Ladder는 청색, 주황색, 녹색으로 염색된 단백질(10-180kDa) 10개로 구성된 혼합물로서 단백질 전기영동(SDS-PAGE)과 웨스턴 블로팅(Western blotting)에서 크기 표준으로 사용할 수 있습니다.

PageRuler Prestained Protein Ladder의 특징:

크기 범위—10-180kDa 범위의 단백질 10개
즉시 사용 가능—겔에 직접 로딩할 수 있도록 Loading buffer로 제공, 끓일 필요 없음
선명한 밴드—유사한 강도의 밴드를 색으로 구분하여 육안으로 쉽게 확인 가능
품질 검사 완료—SDS-PAGE와 웨스턴 블로팅(Western blotting)을 통해 각 로트 평가
참조 밴드 2개—70kDa의 주황색과 10kDa의 녹색
막(Membrane) 호환—웨스턴 블로팅(Western blotting)을 위한 여러 막으로의 컬러 밴드 transfer

이 사전 염색된 단백질 MW 표지자는 SDS-polyacrylamide 겔 전기영동의 진행률을 모니터링하고, PVDF, 나일론, nitrocellulose 막으로의 transfer 효율성을 평가하고, 겔 염색이나 웨스턴 블롯 검출 시약을 통해 육안으로 확인되는 분리된 단백질의 대략적인 크기를 추정하는 데 사용됩니다. 이 래더는 70kDa의 주황색 참조 밴드 1개와 10kDa의 녹색 밴드 1개를 포함합니다.

구성품:
• 62.5mM Tris-H3PO4(25°C에서 pH 7.5)의 염료로 염색된 단백질, 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3, 33% 글리세롤.

어플리케이션:
• SDS-polyacrylamide 겔 전기영동 중에 단백질 이동 모니터링
• 웨스턴 블로팅(Western blotting) 후 막(membrane)으로의 단백질 transfer 모니터링
• SDS-polyacrylamide 겔과 웨스턴 블롯에서 단백질 크기 측정.

관련 자료:
단백질 분자량 표지자—제품 비교 및 선택 가이드
단백질 겔 염색 및 키트—비색 겔 염색과 형광 겔 염색 비교

관련 제품:
Precise Protein Gels—SDS-PAGE용 pre-cast 겔 전체 찾아보기
BupH Dry Blend Electrophoresis Buffer—Tris-HEPES-SDS 및 기타 파우치형 전기영동 버퍼
환원제—2-mercaptoethanol, DTT, TCEP를 비롯한 기타 환원제
웨스턴 블로팅 기질 및 시약—키트, 스트리핑 버퍼(stripping buffer), 트랜스퍼 멤브레인(blotting membrane)과 필름 찾아보기

* 한글화 제품번호: 0026616(NCI6616KR), 용량 : 2 x 250 µL

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
젤 호환성Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
분자량180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa
제품라인PageRuler
제품 유형Protein Ladder
수량2 x 250 μL
로드 준비Yes
배송 조건Approved for shipment on Wet or Dry Ice
염색 유형3 colors: Blue, Orange, Green
시스템 유형Western Blotting, SDS-PAGE
Number of Markers10
크기 범위10 to 180 kDa
Unit SizeEach
구성 및 보관

Contents: two vials of 250 μL each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C

자주 묻는 질문(FAQ)

Why do Thermo Scientific prestained protein ladders not show the real protein sizes?

Coupling of chromophores to proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. The apparent molecular weight of prestained protein standards is calibrated in the classical TRIS glycine-SDS Laemmli system, however prestained proteins may have different mobility in other electrophoresis buffer and gel systems. It should also be noted that the sizing of proteins by gel electrophoresis does not give an exact value and depends on the protein sequence and post-modification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The upper bands of the ladder are missing. What could be the reason?

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the proteins in Thermo Scientific protein ladders have a His-Tag or would otherwise react with an anti-His-Tag antibody?

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can Thermo Scientific protein ladders be detected by Strep-Tactin conjugates?

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why are the PageRuler and Spectra ladder bands detected with antibodies against eukaryotic proteins?

All PageRuler and Spectra ladder bands are recombinant prokaryotic proteins purified from E. coli cells. None of them are related to eukaryotic proteins, however this cannot exclude the possibility that the ladder proteins may possess an epitope that is cross-reactive with the antibody used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.