PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
Thermo Scientific™

PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to자세히 알아보기
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카탈로그 번호수량
266192 x 250 μL
26619X48 x 250 μL
2662010 x 250 μL
카탈로그 번호 26619
제품 가격(KRW)
250,000
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Ends: 31-Dec-2025
277,000
할인액 27,000 (10%)
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수량:
2 x 250 μL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
250,000
Online offer
Ends: 31-Dec-2025
277,000
할인액 27,000 (10%)
Each
카트에 추가하기
Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Plus Prestained Protein Ladder:

Size range—nine proteins spanning 10 to 250kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands—color-coded bands of similar intesity for easy visualization
Quality tested—each lot evaluated by SDS-PAGE and Western blotting
Bright reference bands—orange at 70 and 25kDa, and green at 10kDa
Membrane-compatible—colored bands transfer to membranes for Western blotting

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kDa and 25kDa that are colored with an orange dye and one green reference band of 10kDa. PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required before loading onto a gel.

Includes:
• Dye-stained proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.

Applications:
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after Western blotting
• Sizing of proteins on SDS-polyacrylamide gels and Western blots

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
젤 호환성Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
분자량250, 130, 100, 70, 55, 35, 25, 15, 10 kDa
제품라인PageRuler
제품 유형Protein Ladder
수량2 x 250 μL
로드 준비Yes
배송 조건Approved for shipment on Wet or Dry Ice
염색 유형3 colors: Blue, Orange, Green
시스템 유형Western Blotting, SDS-PAGE
Number of Markers9
크기 범위10 to 250 kDa
Unit SizeEach
구성 및 보관

Contents: two vials of 250 μL each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C

자주 묻는 질문(FAQ)

Why do Thermo Scientific prestained protein ladders not show the real protein sizes?

Coupling of chromophores to proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. The apparent molecular weight of prestained protein standards is calibrated in the classical TRIS glycine-SDS Laemmli system, however prestained proteins may have different mobility in other electrophoresis buffer and gel systems. It should also be noted that the sizing of proteins by gel electrophoresis does not give an exact value and depends on the protein sequence and post-modification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The upper bands of the ladder are missing. What could be the reason?

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the proteins in Thermo Scientific protein ladders have a His-Tag or would otherwise react with an anti-His-Tag antibody?

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can Thermo Scientific protein ladders be detected by Strep-Tactin conjugates?

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why are the PageRuler and Spectra ladder bands detected with antibodies against eukaryotic proteins?

All PageRuler and Spectra ladder bands are recombinant prokaryotic proteins purified from E. coli cells. None of them are related to eukaryotic proteins, however this cannot exclude the possibility that the ladder proteins may possess an epitope that is cross-reactive with the antibody used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.