CaptureSelect™ Factor H Affinity Matrix

CaptureSelect Factor H Affinity Matrix is designed for the purification of human complement Factor H from plasma and recombinant sources.

Features of the CaptureSelect Factor H Affinity Matrix include:

• Highly selective for human Factor H with no affinity for other human plasma proteins
• Binds both plasma-derived and recombinant full-length Factor H
• Enables efficient purification of Factor H with high yields and retained activity
• Efficient elution at acidic pH
• Excellent scalability
• Non-animal-derived

The high selectivity and yields obtained using CaptureSelect Factor H Affinity Matrix enable a robust, fast, and efficient purification process with excellent purity in one step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created using a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

Matrix: agarose-based

Average particle size: 65 ± 10 μm

Ligand: CaptureSelect Factor H affinity ligand

Ligand coupling method: epoxide

Binding capacity: > 2 mg Factor H/mL resin

Binding conditions: PBS or TBS, pH 7

Elution conditions: 20 mM citric acid, pH 3

Flow characteristics: 50–200 cm/h (up to 2 bar)

Formulation buffer: 20% (v/v) ethanol