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인용 및 참조 문헌 (2)
Gibco™
DMEM, high glucose, no glutamine, no phenol red
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 수량 |
|---|---|
| 31053028 | 500 mL |
| 31053036 | 10 x 500 mL |
카탈로그 번호 31053028
제품 가격(KRW)
60,000
キャンペーン価格
Ends: 31-Dec-2025
63,000할인액 3,000 (5%)
Each
수량:
500 mL
제품 가격(KRW)
60,000
キャンペーン価格
Ends: 31-Dec-2025
63,000할인액 3,000 (5%)
Each
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
This DMEM is modified as follows:
| With | Without |
| • High Glucose | • L-glutamine |
| • Phenol Red | |
| • Sodium Pyruvate | |
| • HEPES |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포주HeLa, 293, Cos-7, and PC-12
세포 유형Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
농도1 X
Glucose 농도4500 mg/L
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형DMEM (Dulbecco's Modified Eagle Medium)
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Liquid
Serum LevelStandard Serum Supplementation
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함High Glucose
첨가제 없음No Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
자주 묻는 질문(FAQ)
Do you offer DMEM, high glucose without phenol red?
What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?
I understand that some media are worse than others for fluorescence imaging. How do I choose?
Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?
How long can I keep my media after supplementing with serum?
인용 및 참조 문헌 (2)
인용 및 참조 문헌
Abstract
Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy: isolation of smooth muscle and endothelial cells from mouse brain.
Journal:Methods Mol Biol
PubMed ID:22528096
'The use of murine cerebrovascular endothelial and smooth muscle cells has not been widely employed as a cell culture model for the investigation of cellular mechanisms involved in cerebral amyloid angiopathy (CAA). Difficulties in isolation and propagation of murine cerebrovascular cells and insufficient yields for molecular and cell culture studies
Potentially novel candidate biomarkers for head and neck squamous cell carcinoma identified using an integrated cell line-based discovery strategy.
Journal:Mol Cell Proteomics
PubMed ID:22918226
Head and neck squamous cell carcinomas (HNSCC) can arise from the oral cavity, oropharynx, larynx or hypopharynx, and is the sixth leading cancer by incidence worldwide. The 5-year survival rate of HNSCC patients remains static at 40-60%. Hence, biomarkers which can improve detection of HNSCC or early recurrences should improve