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인용 및 참조 문헌 (1)
Gibco™
RPMI 1640 Medium, no glutamine
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 31870025 | 500 mL |
카탈로그 번호 31870025
제품 가격(KRW)
55,000
Each
수량:
500 mL
제품 가격(KRW)
55,000
Each
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Life Technologies offers a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Product intended use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers an identical RPMI 1640 product made in our Grand Island facility (21870-092). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
This RPMI is modified as follows:
| With | Without | |||
| • Phenol Red | • HEPES | |||
| • L-glutamine |
The complete formulation is available.
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Product intended use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers an identical RPMI 1640 product made in our Grand Island facility (21870-092). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포 유형Mammalian Cells
농도1 X
배양 환경CO2
제조 품질cGMP-compliant under the ISO 13485 standard
삼투압260 - 310 mOsm/kg
제품라인Gibco
제품 유형RPMI 1640
수량500 mL
유통 기한12 Months
배송 조건Room Temperature
분류Animal Origin-free
Culture TypeMammalian Cell Culture
형태Liquid
Serum LevelStandard Serum Supplementation
멸균Sterile-filtered
첨가제 포함Phenol Red, Sodium Bicarbonate
첨가제 없음No Glutamine, No HEPES, No Sodium Pyruvate
pH7 to 7.4
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
자주 묻는 질문(FAQ)
How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?
What is the density (g/L) for RPMI 1640 Medium?
인용 및 참조 문헌 (1)
인용 및 참조 문헌
Abstract
Tracing fatty acid metabolism by click chemistry.
Journal:ACS Chem Biol
PubMed ID:22999348
Fatty acids are abundant constituents of all biological systems, and their metabolism is important for normal function at all levels of an organism. Aberrations in fatty acid metabolism are associated with pathological states and have become a focus of current research, particularly due to the interest in metabolic overload diseases.