Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced Serum Medium
인용 및 참조 문헌 (5)
Gibco™

Opti-MEM™ I Reduced Serum Medium

Opti-MEM™ I Reduced-Serum Medium은 개선된 Minimal Essential Medium(MEM)으로 소 태아 혈청(Fetal Bovine Serum) 첨가량을 성장 속도나 형태학적 변화 없이 적어도자세히 알아보기
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카탈로그 번호수량
3198508810 x 500 mL
31985070500 mL
31985062100 mL
카탈로그 번호 31985088
제품 가격(KRW)
731,000
Online offer
Ends: 31-Dec-2025
769,000
할인액 38,000 (5%)
Each
카트에 추가하기
수량:
10 x 500 mL
Customize this product
제품 가격(KRW)
731,000
Online offer
Ends: 31-Dec-2025
769,000
할인액 38,000 (5%)
Each
카트에 추가하기
Opti-MEM™ I Reduced-Serum Medium은 개선된 Minimal Essential Medium(MEM)으로 소 태아 혈청(Fetal Bovine Serum) 첨가량을 성장 속도나 형태학적 변화 없이 적어도 50% 이상 절감할 수 있습니다. Opti-MEM™ I 배지는 또한 Lipofectamine™ 시약과 같은 양이온 지질 형질주입 시약과 함께 사용하는 것이 권장됩니다. Opti-MEM™ I 배지는 Sp2, AE-1, CHO, BHK-21, HEK, 일차 섬유아세포(primary fibroblast) 등 다양한 현탁 및 부착 포유류 세포에 사용할 수 있습니다. 당사는 다양한 조성의 Opti-MEM™ I 제품으로 광범위한 세포 배양 응용 분야를 지원합니다.

이 Opti-MEM™ I은 다음과 같이 조성이 변경됩니다.
포함:
• L-글루타민
• 페놀 레드(phenol red)

전체 조성은 비공개 사항입니다. 자세한 내용은 기술 서비스팀에문의하십시오.

Opti-MEM™ I 배지의 사용
Opti-MEM™ I Reduced-Serum Medium은 인슐린, 트랜스페린(transferrin), 하이포크산틴(hypoxanthine), 티미딘(thymidine) 및 미량 원소를 포함하는 고유한 배지입니다. 이러한 추가 성분으로 혈청 첨가량을 적어도 50% 만큼 줄일 수 있습니다. Opti-MEM™ I 배지는 중탄산나트륨(sodium bicarbonate) 버퍼 시스템(2.4 g/L)을 이용하며, 이로 인해 생리적학적 pH를 유지하기 위해 5–10% CO2 환경이 요구됩니다.

cGMP 제조 및 품질 시스템
Opti-MEM™ I는 영국 스코틀랜드의 Paisley에 위치한 cGMP 규정 준수 시설에서 제조됩니다. 이 시설은 의료기기 제조업체로 FDA에 등록되어 있으며 ISO 13485 스탠더드 인증을 받았습니다. 공급망 유지를 위해 당사는 또한 Grand Island 시설에서 동일한 Opti-MEM™ I 제품을 제조하여 공급합니다(31985-070). 이 시설도 또한 의료기기 제조업체로 FDA에 등록되어 있으며 ISO 13485 스탠더드 인증을 받았습니다.

This product is not used for in vitro diagnostic purpose in some countries.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포주Sp2, AE-1, CHO, BHK-21, and HEK
세포 유형Primary Fibroblasts
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco, Opti-MEM
제품 유형Opti-MEM I
수량10 x 500 mL
유통 기한18 Months From Date of Manufacture
배송 조건Room Temperature
분류Human Origin
형태Liquid
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함Glutamine, Phenol Red
Unit SizeEach
구성 및 보관
Store at 2–8°C and protect from light.

자주 묻는 질문(FAQ)

What should I use to dilute my RNAi duplexes and Lipofectamine RNAiMAX before complexing?

We recommend using Opti-MEM I Reduced Serum Medium (Cat. No. 31985062).

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Do you offer an alternative for Opti-MEM I Reduced Serum Medium (Cat. No. 31985070)?

Opti-MEM I Reduced Serum Medium, no phenol red (Cat. No. 11058021) would be the closest alternative. Both offerings contain L-glutamine. For more a stabilized L-glutamine option, Opti-MEM I Reduced Serum Medium, GlutaMAX Supplement (Cat. No. 51985034) is the recommended alternative. All Opti-MEM offerings have demonstrated performance for both reduced-serum cell culture and lipid-based transfection.

My OpitMEM Reduced Serum Medium (Cat. No. 31985070) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Opti-MEM™ I Reduced Serum Medium, 10 x 500 mL FAQs

인용 및 참조 문헌 (5)

인용 및 참조 문헌
Abstract
Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.
Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; Böhm Michael; Nickenig Georg;
Journal:Circulation
PubMed ID:12021231
BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More
Regulation of cortical dendrite development by Slit-Robo interactions.
Authors:Whitford Kristin L; Marillat Valérie; Stein Elke; Goodman Corey S; Tessier-Lavigne Marc; Chédotal Alain; Ghosh Anirvan;
Journal:Neuron
PubMed ID:11779471
Slit proteins have previously been shown to regulate axon guidance, branching, and neural migration. Here we report that, in addition to acting as a chemorepellant for cortical axons, Slit1 regulates dendritic development. Slit1 is expressed in the developing cortex, and exposure to Slit1 leads to increased dendritic growth and branching. ... More
Cloning and characterization of freac-9 (FKHL17), a novel kidney-expressed human forkhead gene that maps to chromosome 1p32-p34.
Authors: Ernstsson S; Betz R; Lagercrantz S; Larsson C; Ericksson S; Cederberg A; Carlsson P; Enerbäck S;
Journal:Genomics
PubMed ID:9403061
'We describe the cloning of a near full-length cDNA of 4258 nucleotides encoding freac-9 (HGMW-approved symbol FKHL17), a novel human forkhead gene. The 5'' untranslated region is unusual since it is very long, 2127 nucleotides, and contains 15 upstream AUG codons. Hybridization to a panel consisting of RNA derived from ... More
Noninfectious virus-like particles produced by Moloney murine leukemia virus-based retrovirus packaging cells deficient in viral envelope become infectious in the presence of lipofection reagents.
Authors:Sharma S, Murai F, Miyanohara A, Friedmann T
Journal:Proc Natl Acad Sci U S A
PubMed ID:9380714
Retrovirus packaging cell lines expressing the Moloney murine leukemia virus gag and pol genes but lacking virus envelope genes produce virus-like particles constitutively, whether or not they express a transcript from an integrated retroviral provirus. In the absence of a proviral transcript, the assembled particles contain processed gag and reverse ... More
Human T-cell leukemia virus type I Tax protein transactivates RNA polymerase III promoter in vitro and in vivo.
Authors: Piras G; Dittmer J; Radonovich M F; Brady J N;
Journal:J Biol Chem
PubMed ID:8702791
Tax protein of the human T-cell lymphotropic virus type 1 (HTLV-I) is critical for viral replication and is a potent transcriptional activator of viral and cellular polymerase II (pol II) genes. We report here that Tax is able to transactivate a classical pol III promoter, VA-I. In cotransfection experiments, Tax ... More