Pierce™ ECL Western Blotting Substrate
Pierce™ ECL Western Blotting Substrate
Thermo Scientific™

Pierce™ ECL Western Blotting Substrate

Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that자세히 알아보기
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호수량
32106500 mL
3210950 mL
32209X44 x 250 mL
32209250 mL
32106X44 x 500 mL
32106TS
32106으로도 사용됨
카탈로그 번호 32106
제품 가격(KRW)
382,000
キャンペーン価格
Ends: 31-Dec-2025
424,000
할인액 42,000 (10%)
Each
카트에 추가하기
수량:
500 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
382,000
キャンペーン価格
Ends: 31-Dec-2025
424,000
할인액 42,000 (10%)
Each
카트에 추가하기
Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce ECL Plus Western Blotting Substrate characteristics include:
Sensitivity: low picogram
Stability: 1-hr working solution stability, 1-year kit stability at 4°C
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 1 to 2 hours
Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 μg/mL)
Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 μg/mL)

Compare and learn more about our western blot chemiluminescent substrates ›
View all HRP substrates available ›

Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.

Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.

For Research Use Only. Not for use in diagnostic procedures.
사양
콘주게이트HRP
설명Pierce ECL Western Blotting Substrate
키트 구성품Luminol/Enhancer, 250mL, Stable Peroxide Buffer, 250mL
권장 항체 농도2°: 1:1-15K (0.07-1μg/mL), 1°: 1:1K (0.2- 10μg/mL)
민감도Low Picogram
신호 지속 시간1 to 2 hr.
충분5000 sq. cm
검출 방법Chemiluminescence
용도(애플리케이션)Western Blot
형태Liquid
Membrane CompatibilityNitrocellulose, PVDF
제품라인Pierce
제품 유형Substrate
수량500 mL
SubstrateHorseradish Peroxidase
Unit SizeEach
구성 및 보관
Upon receipt store at 2-8°C. Product is shipped at ambient temperature.

자주 묻는 질문(FAQ)

How much more sensitive is SuperSignal West Femto Maximum Sensitivity Substrate than the other enhanced chemiluminescent (ECL) substrates?

SuperSignal West Femto Maximum Sensitivity Substrate is about 25- to 50-fold more sensitive than GE Healthcare ECL Substrate or Thermo Scientific ECL Substrate (Cat. No. 32106). SuperSignal West Femto Maximum Sensitivity Substrate is also significantly more sensitive than available chemiluminescent substrates for alkaline phosphatase. Please also see Tech Tip # 32 Guide to Enzyme Substrates for Western Blotting (https://tools.thermofisher.com/content/sfs/brochures/TR0032-Substrates-Western.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How much more sensitive is SuperSignal West Dura Extended Duration Substrate than the other enhanced chemiluminescent (ECL) substrates?

SuperSignal West Dura Extended Duration Substrate is about 10-fold more sensitive than GE Healthcare ECL Substrate or Thermo Scientific ECL Substrate (Cat. No. 32106). SuperSignal West Dura Extended Duration Substrate is also significantly more sensitive than available chemiluminescent substrates for alkaline phosphatase. Please also see Tech Tip: Guide to Enzyme Substrates for Western Blotting (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0032-Substrates-Western.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.