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Thermo Scientific™
Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
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보기 방식 변경
| 카탈로그 번호 | 수량 |
|---|---|
| 32132 | 100 mL Kit |
| 32134 | 25 mL Kit |
| 32132X3 | 300 mL Kit |
카탈로그 번호 32132
제품 가격(KRW)
522,000
Online offer
Ends: 31-Mar-2026
580,000할인액 58,000 (10%)
Each
수량:
100 mL Kit
제품 가격(KRW)
522,000
Online offer
Ends: 31-Mar-2026
580,000할인액 58,000 (10%)
Each
Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.
Features of ECL Plus Substrate:
- Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
- Higher sensitivity—detect targets down to the low-picogram level
- Longer signal duration—sustained light output for as long as 5 hours
- More imaging options—X-ray, CCD or laser-based imagers
- More affordable—high quality and performance at a lower price than other suppliers' similar substrates
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
For Research Use Only. Not for use in diagnostic procedures.
사양
콘주게이트HRP
설명Pierce ECL Plus Western Blotting Substrate
키트 구성품Detection Reagent A, 100 mL, Detection Reagent B, 2.5 mL
민감도Low picogram to mid-femtogram
충분10 mini-gel blots, 1000 cm2 of membrane
검출 방법Chemiluminescence, Fluorescence
용도(애플리케이션)Protein Assay and Analysis
형태Liquid
Membrane CompatibilityNitrocellulose, PVDF
제품라인Pierce
제품 유형Substrate
수량100 mL Kit
Unit SizeEach
구성 및 보관
Upon receipt store at 4°C. Product shipped on ice.