SuperSignal™ West Dura Extended Duration Substrate
SuperSignal™ West Dura Extended Duration Substrate
Thermo Scientific™

SuperSignal™ West Dura Extended Duration Substrate

Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for자세히 알아보기
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카탈로그 번호수량
34076200 mL
34075100 mL
34075X44 x 100 mL
34076X44 x 200 mL
3707120 mL
카탈로그 번호 34076
제품 가격(KRW)
1,106,000
Each
카트에 추가하기
수량:
200 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
1,106,000
Each
카트에 추가하기
Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal West Dura Extended Duration Substrate characteristics include:
Sensitivity: mid-femtogram
Stability: 24-hr working solution stability; 1-year kit stability at room temperature
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 24 hours
Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

Compare and view all western blot chemiluminescent substrates ›

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, allowing multiple film or camera exposures.
For Research Use Only. Not for use in diagnostic procedures.
사양
키트 구성품Luminol/Enhancer, 100 mL, Stable Peroxide Buffer, 100 mL
권장 항체 농도2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1μg/mL)
민감도Mid-femtogram
신호 지속 시간24 hr.
충분25 mini-gel blots, 2000 sq. cm of membrane
검출 방법Chemiluminescence
용도(애플리케이션)Western Blot
형태Liquid
Membrane CompatibilityNitrocellulose, PVDF
제품라인SuperSignal
제품 유형Substrate
수량200 mL
SubstrateHorseradish Peroxidase
Unit SizeEach
구성 및 보관
Sufficient For: 25 mini-gel blots; 2000 cm2 of membrane
• Luminol/Enhancer, 100 mL
• Stable Peroxide Buffer, 100 mL

Store at room temperature.

자주 묻는 질문(FAQ)

What types of membrane work best with SuperSignal West Dura Extended Duration Substrate?

Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Dura Extended Duration Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will SuperSignal West Dura Extended Duration Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Dura Extended Duration Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used the SuperSignal West Dura Extended Duration Substrate. Why is the entire film black or showing a reversed image?

The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why do I see more bands with the SuperSignal West Dura Extended Duration Substrate than I've seen in the past with other substrates?

Because this substrate is more sensitive than other chemiluminescent substrates you might detect low-abundance proteins that were not visible before. When using a more sensitive substrate, more careful optimization of blocking buffer steps and antibody concentrations is required.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What protocol should I use to strip and reprobe a blot detected with SuperSignal West Dura Extended Duration Substrate?

See Tech Tip # 23: Strip and reprobe Western blots.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.