SuperSignal™ West Dura Extended Duration Substrate
Thermo Scientific™
SuperSignal™ West Dura Extended Duration Substrate
Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for자세히 알아보기
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수량
34076
200 mL
34075
100 mL
34075X4
4 x 100 mL
34076X4
4 x 200 mL
37071
20 mL
5 개 옵션
카탈로그 번호 34076
제품 가격(KRW)
1,106,000
Each
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수량:
200 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
1,106,000
Each
카트에 추가하기
Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.
SuperSignal West Dura Extended Duration Substrate characteristics include: • Sensitivity: mid-femtogram • Stability: 24-hr working solution stability; 1-year kit stability at room temperature • Compatibility: nitrocellulose and PVDF membranes • Signal duration: 24 hours • Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL) • Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)
SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, allowing multiple film or camera exposures.
For Research Use Only. Not for use in diagnostic procedures.
사양
키트 구성품Luminol/Enhancer, 100 mL, Stable Peroxide Buffer, 100 mL
Sufficient For: 25 mini-gel blots; 2000 cm2 of membrane • Luminol/Enhancer, 100 mL • Stable Peroxide Buffer, 100 mL
Store at room temperature.
자주 묻는 질문(FAQ)
What types of membrane work best with SuperSignal West Dura Extended Duration Substrate?
Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Dura Extended Duration Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).
Will SuperSignal West Dura Extended Duration Substrate work with nucleic acid blots (Southern blotting, etc.)?
Yes. However, SuperSignal West Dura Extended Duration Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).
I used the SuperSignal West Dura Extended Duration Substrate. Why is the entire film black or showing a reversed image?
The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.
Why do I see more bands with the SuperSignal West Dura Extended Duration Substrate than I've seen in the past with other substrates?
Because this substrate is more sensitive than other chemiluminescent substrates you might detect low-abundance proteins that were not visible before. When using a more sensitive substrate, more careful optimization of blocking buffer steps and antibody concentrations is required.