SuperSignal™ West Femto Maximum Sensitivity Substrate
SuperSignal™ West Femto Maximum Sensitivity Substrate
Thermo Scientific™

SuperSignal™ West Femto Maximum Sensitivity Substrate

Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate는 horseradish peroxidase(HRP) 효소를 사용한 웨스턴 블로팅 분석을 통한 낮은 펨토그램 수준 검출을자세히 알아보기
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카탈로그 번호수량
34095100 mL Kit
3409420 mL Kit
34095X44 x 100 mL
34096X44 x 200 mL
34096200 mL Kit
카탈로그 번호 34095
제품 가격(KRW)
718,000
Each
카트에 추가하기
수량:
100 mL Kit
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
718,000
Each
카트에 추가하기
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate는 horseradish peroxidase(HRP) 효소를 사용한 웨스턴 블로팅 분석을 통한 낮은 펨토그램 수준 검출을 위한 초고감도 ECL(Enhanced chemilescent) HRP 기질입니다.

SuperSignal West Femto Maximum Sensitivity Substrate의 특성은 다음과 같습니다.
• 감도: 낮은 펨토그램
• 안정성: 8시간 작업 용액 안정성, 4°C에서 1년 키트 안정성
• 호환성: 니트로셀룰로오스 및 PVDF 멤브레인
• 신호 지속 시간: 최대 8시간
• 권장 1차 항체 농도: 1:5,000–1:100,000 희석(10–200 ng/mL)
• 권장 2차 항체 농도: 1:100,000–1:500,000 희석(2–10ng/mL)

당사의 웨스턴 블로팅 화학 발광 기질 비교 및 자세히 알아보기 ›
모든 HRP 기질 보기 사용 가능›

SuperSignal West Femto Maximum Sensitivity Substrate는 웨스턴 블로팅 분석을 통한 낮은 펨토그램 수준 검출용으로 설계되었습니다. 최적화된 항체 농도 및 차단 완충액과 결합할 경우 SuperSignal West Femto Substrate를 사용하면 일반적인 ECL 기질로는 극소량으로 보이는 표적 단백질을 검출할 수 있습니다.

For Research Use Only. Not for use in diagnostic procedures.
사양
키트 구성품Luminol/Enhancer, 50mL, Stable Peroxide Buffer, 50mL
권장 항체 농도2° 1:100 ∼ 500K(2 ∼ 10ng/mL), 1°: 1:5K(10 ∼ 200ng/mL)
민감도Low femtogram-level
신호 지속 시간6 ∼ 8시간
충분12 Mini-Gel Blots, 1000 sq. cm of Membrane
검출 방법화학 발광
용도(애플리케이션)Western Blot
형태액체
Membrane CompatibilityNitrocellulose, PVDF
제품라인SuperSignal
제품 유형기질
수량100 mL Kit
SubstrateHorseradish Peroxidase
Unit SizeEach
구성 및 보관
다음에 대해 충분함: 12 mini-gel blot, 멤브레인 1000 cm2
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

4°C에 시약 보관

자주 묻는 질문(FAQ)

What types of membrane work best with SuperSignal West Femto Maximum Sensitivity Substrate?

Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Femto Maximum Sensitivity Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will SuperSignal West Femto Maximum Sensitivity Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Femto Maximum Sensitivity Substrate was optimized for use in Western blot sand is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used the SuperSignal West Femto Maximum Sensitivity Substrate. Why is the entire film black or showing a reversed image?

The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why do I see more bands with the SuperSignal West Femto Maximum Sensitivity Substrate than I've seen in the past with other substrates?

Because this substrate is more sensitive than other chemiluminescent substrates you might detect low-abundance proteins that were not visible before. When using a more sensitive substrate, more careful optimization of blocking buffer steps and antibody concentrations is required.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What protocol should I use to strip and reprobe a blot detected with SuperSignal West Femto Maximum Sensitivity Substrate ?

See Tech Tip # 23: Strip and reprobe Western blots.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all SuperSignal™ West Femto Maximum Sensitivity Substrate FAQs