SuperSignal™ West Femto Maximum Sensitivity Substrate
Thermo Scientific™
SuperSignal™ West Femto Maximum Sensitivity Substrate
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate는 horseradish peroxidase(HRP) 효소를 사용한 웨스턴 블로팅 분석을 통한 낮은 펨토그램 수준 검출을자세히 알아보기
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보기 방식 변경
카탈로그 번호
수량
34095
100 mL Kit
34094
20 mL Kit
34095X4
4 x 100 mL
34096X4
4 x 200 mL
34096
200 mL Kit
5 개 옵션
카탈로그 번호 34095
제품 가격(KRW)
718,000
Each
카트에 추가하기
수량:
100 mL Kit
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
718,000
Each
카트에 추가하기
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate는 horseradish peroxidase(HRP) 효소를 사용한 웨스턴 블로팅 분석을 통한 낮은 펨토그램 수준 검출을 위한 초고감도 ECL(Enhanced chemilescent) HRP 기질입니다.
SuperSignal West Femto Maximum Sensitivity Substrate의 특성은 다음과 같습니다. • 감도: 낮은 펨토그램 • 안정성: 8시간 작업 용액 안정성, 4°C에서 1년 키트 안정성 • 호환성: 니트로셀룰로오스 및 PVDF 멤브레인 • 신호 지속 시간: 최대 8시간 • 권장 1차 항체 농도: 1:5,000–1:100,000 희석(10–200 ng/mL) • 권장 2차 항체 농도: 1:100,000–1:500,000 희석(2–10ng/mL)
SuperSignal West Femto Maximum Sensitivity Substrate는 웨스턴 블로팅 분석을 통한 낮은 펨토그램 수준 검출용으로 설계되었습니다. 최적화된 항체 농도 및 차단 완충액과 결합할 경우 SuperSignal West Femto Substrate를 사용하면 일반적인 ECL 기질로는 극소량으로 보이는 표적 단백질을 검출할 수 있습니다.
For Research Use Only. Not for use in diagnostic procedures.
다음에 대해 충분함: 12 mini-gel blot, 멤브레인 1000 cm2 • Luminol/Enhancer, 50 mL • Stable Peroxide Buffer, 50 mL
4°C에 시약 보관
자주 묻는 질문(FAQ)
What types of membrane work best with SuperSignal West Femto Maximum Sensitivity Substrate?
Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Femto Maximum Sensitivity Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).
Will SuperSignal West Femto Maximum Sensitivity Substrate work with nucleic acid blots (Southern blotting, etc.)?
Yes. However, SuperSignal West Femto Maximum Sensitivity Substrate was optimized for use in Western blot sand is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).
I used the SuperSignal West Femto Maximum Sensitivity Substrate. Why is the entire film black or showing a reversed image?
The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.
Why do I see more bands with the SuperSignal West Femto Maximum Sensitivity Substrate than I've seen in the past with other substrates?
Because this substrate is more sensitive than other chemiluminescent substrates you might detect low-abundance proteins that were not visible before. When using a more sensitive substrate, more careful optimization of blocking buffer steps and antibody concentrations is required.