Pierce™ Fast Western Blot Kit, ECL Substrate
Pierce™ Fast Western Blot Kit, ECL Substrate
Thermo Scientific™

Pierce™ Fast Western Blot Kit, ECL Substrate

The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time required to perform자세히 알아보기
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카탈로그 번호수량
35055200 mL Kit
350501 L Kit
카탈로그 번호 35055
제품 가격(KRW)
217,000
Online offer
Ends: 31-Dec-2025
255,000
할인액 38,000 (15%)
Each
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수량:
200 mL Kit
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
217,000
Online offer
Ends: 31-Dec-2025
255,000
할인액 38,000 (15%)
Each
카트에 추가하기
The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Features of this kit:

Fast—all the sensitivity of Pierce ECL substrate and saving 4 to 5 hours per blot
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—cost as little as $8 per blot; no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kit gives you all the components you need to block and probe and develop a blot with your mouse or rabbit primary antibody

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. The protocol is a quick, efficient and economic way to obtain Western blot results without the hassle of buying an instrument and the consumables required by other popular rapid Western blotting systems. No vacuum pump is needed so there are never any clogged lines or membranes that can occur when using instrument-based systems. Because there is no apparatus, there is no need to buy disposable blotting trays that limit the number of blots processed at one time.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
콘주게이트HRP
반응성Mouse, Rabbit
충분5 Mini Blots
검출 방법Chemiluminescence
용도(애플리케이션)Western Blot
형태Liquid
Membrane CompatibilityNitrocellulose, PVDF
제품라인Pierce
제품 유형Western Blot Kit
수량200 mL Kit
SubstrateHorseradish Peroxidase
Unit SizeEach
구성 및 보관
Sufficient For: 5 mini blots probed with mouse or rabbit primary antibodies
• Fast Western Antibody Diluent, 100 mL
• Fast Western 10X Wash Buffer, 50 mL
• Fast Western Optimized HRP Reagent, 5 mL
• Pierce ECL Detection Reagent 1, 25 mL
• Pierce ECL Detection Reagent 2, 25 mL

Store at 4°C.

자주 묻는 질문(FAQ)

Does my primary antibody need to be conjugated for using with the Pierce Fast Western Blot Kit, ECL Substrate (Cat. No. 35050, 35055)?

No, your primary antibody does not need to be conjugated for using with the Pierce Fast Western Blot Kit, ECL Substrate (cat. No. 35050, 35055). The kit includes all necessary reagents except the primary antibody, and it works with both mouse and rabbit primary antibodies. The HRP reagent directly binds to both mouse and rabbit primary antibodies, thus, a secondary antibody is not needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

For western blotting with the Pierce Fast Western Blot Kit, ECL Substrate (Cat. No. 35050), do I need a secondary antibody?

The kit contains the horseradish peroxidase (HRP) reagent and ECL detection reagent, therefore the kit works with blots probed with mouse or rabbit primary antibodies. The HRP reagent directly binds to both mouse or rabbit primary antibodies, thus, a secondary antibody is not needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.