Fish Serum Blocking Buffer
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Fish Serum Blocking Buffer
Thermo Scientific™

Fish Serum Blocking Buffer

Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalian samples.
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카탈로그 번호수량
37527X33 x 500 mL
37527500 mL
카탈로그 번호 37527X3
제품 가격(KRW)
702,000
온라인 행사
Ends: 31-Dec-2025
825,000
할인액 123,000 (15%)
Each
카트에 추가하기
수량:
3 x 500 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
702,000
온라인 행사
Ends: 31-Dec-2025
825,000
할인액 123,000 (15%)
Each
카트에 추가하기
Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalian samples. Fish Serum Blocking Buffer is effective at stabilizing solutions and samples used for antibody-binding interactions, while at the same time minimizing the possibility of cross-reaction with mammalian sample components.
Features of Fish Serum Blocking Buffer include:
Non-mammalian—fish proteins are less likely to have specific binding interactions with antibodies and other mammalian proteins present in typical methods
Convenient—filtered and stabilized in PBS for compatibility with most assay systems
Easy to use—can be used as supplied or diluted up to 10-fold as needed
Flexible - may be used for many different applications, including as a diluent for antibodies

Fish serum offers several advantages over typical blocking buffers. Because salmon is phylogenetically distant from mammals, which are the source of the antibodies and samples used in most experiments, its serum proteins are less likely to have specific binding interactions with proteins used in the experiments. Fish Serum Blocking Buffer is effective at stabilizing solutions and samples used for antibody-binding interactions while at the same time minimizing the possibility of cross-reaction with mammalian sample components. Try it when use of more-standard sera results in background staining in IHC. In addition, this blocker is effective in various cellular imaging protocols based on visible and near-infrared fluorescence detection. It has been used to decrease background and increase signal-to-noise ratios with near-IR fluorescent probes.

For Research Use Only. Not for use in diagnostic procedures.
  1. Alegria-Schaffer, A., et al. (2009). Performing and optimizing Western blots with an emphasis on chemiluminescent detection. Methods Enzymol. 463:573-99.
  2. Hypolite, J.A., et al. (2001). Am. J. Physiol. Cell. Physiol. 280:C254-64.
  3. Wang, L, et al. (2002). J. Clin. Invest. 110:1175-1184.
사양
화학물질 이름 또는 재질Fish Serum Blocking Buffer
권장 스토리지Upon receipt store at 4°C.
농도1X
용도(애플리케이션)ELISA
물리적 형태Liquid
수량3 x 500 mL
Unit SizeEach

자주 묻는 질문(FAQ)

What is the concentration of protein in the Fish Serum Blocking Buffer?

This information is proprietary. The protein concentration in the Fish Serum Blocking Buffer has been optimized for direct usage without further dilution, however if desired, the blocking buffer may be diluted with phosphate-buffered saline. For best results, our recommendation is to empirically determine the optimal concentration to use.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.