StartingBlock™ Blocking Buffer
StartingBlock™ Blocking Buffer
Thermo Scientific™

StartingBlock™ Blocking Buffer

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays.
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카탈로그 번호수량화학물질 이름 또는 재질
375391 LPBST
37578100 mLPBS
375381 LPBS
37579100 mLTBS
37538X33 x 1 LPBS
375421 LTBS
375431 LTBST
카탈로그 번호 37539
제품 가격(KRW)
381,000
Each
카트에 추가하기
수량:
1 L
화학물질 이름 또는 재질:
PBST
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
381,000
Each
카트에 추가하기
StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock Blocking Buffer is available in PBS and TBS, with and without Tween 20.
Features of StartingBlock Blocking Buffer include:
Strip and re-probe without re-blocking—blots stay blocked even after stripping with Restore Stripping Buffer (Cat. No. 21059)
Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum- and biotin-free)
Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes; almost instantaneous for polystyrene microplate wells

For Research Use Only. Not for use in diagnostic procedures.
사양
화학물질 이름 또는 재질PBST
제제Proprietary protein formulation in phosphate-buffered saline, pH 7.5, with 0.05% Tween 20
권장 스토리지Upon receipt store at 4°C.
농도1X
용도(애플리케이션)Western Blot
물리적 형태Liquid
제품라인StartingBlock
수량1 L
Unit SizeEach

자주 묻는 질문(FAQ)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.