Pierce™ Fast Blocking Buffer
Thermo Scientific™

Pierce™ Fast Blocking Buffer

Optimize Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents with Thermo Scientific™ Pierce Fast Blocking Buffer, a ready-to-use blocker solution.
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카탈로그 번호수량
37576100 mL
37575500 mL
카탈로그 번호 37576
제품 가격(KRW)
240,000
온라인 행사
Ends: 30-Jun-2026
274,000
할인액 34,000 (12%)
Each
수량:
100 mL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
240,000
온라인 행사
Ends: 30-Jun-2026
274,000
할인액 34,000 (12%)
Each

Pierce Fast Blocking Buffer effectively and reliably blocks western blots in just five minutes to provide low-background results for traditional western blotting protocols.

Pierce Fast Blocking Buffer streamlines western blot protocols by providing consistent, high-quality blocking for nitrocellulose or PVDF membranes in only five minutes, producing results comparable to traditional blockers. Blocking and probing steps with Fast Blocking Buffer prevent nonspecific binding of detection antibodies during probing steps but allows specific detection to occur. Pierce Fast Wash Buffer provides efficient membrane washing after incubation with a primary and secondary antibody to effectively shorten total washing time to 20 minutes (from the typical 90 minutes). This buffer is compatible with antibodies and biotin-avidin systems, as well as both nitrocellulose and PVDF membranes.

Related products

Pierce Fast Wash Buffer, 10X (Cat. No. 37577)

For Research Use Only. Not for use in diagnostic procedures.
사양
화학물질 이름 또는 재질Blocking Buffer
권장 스토리지Upon receipt store at 4°C.
농도1X
용도(애플리케이션)Western Blot
물리적 형태Liquid
수량100 mL
Unit SizeEach

자주 묻는 질문(FAQ)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.