NCTC-135 Medium
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Gibco™

NCTC-135 Medium

NCTC-135 medium is a chemically defined medium that was originally developed at the National Cancer Institute (Bethesda, MD) and formulated자세히 알아보기
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카탈로그 번호수량
41350026500 mL
카탈로그 번호 41350026
제품 가격(KRW)
189,000
Each
카트에 추가하기
수량:
500 mL
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제품 가격(KRW)
189,000
Each
카트에 추가하기

NCTC-135 medium is a chemically defined medium that was originally developed at the National Cancer Institute (Bethesda, MD) and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Gibco™ NCTC-135 medium has also been used to grow many cells, including HeLa, Ehrlich-Lettre Ascites strain E, NCTC clone 1469, NCTC 4206, mouse C3H, BS-C-1, and hybridoma cell lines.

This NCTC-135 is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red 
• Coenzymes 


The complete formulation is available.

Gibco™ NCTC-135 is a modification of NCTC 109 medium that replaced L-cysteine with L-cystine, which reduced toxicity to some cells. NCTC-135 medium is rich in nucleosides contains several unique components to eliminate the need for serum, including coenzymes, glucosamine, reducing agents and additional vitamins.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

NCTC-135 medium contains no proteins, lipids, or growth factors. NCTC-135 medium was designed for use without serum for L929 cells, but typically requires supplementation of a growth factor cocktail or Fetal Bovine Serum (FBS) for other cells. NCTC-135 medium uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Some cell lines might require gradual adaptation to a serum-free medium.

For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Mammalian Cells
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형NCTC-135 Medium
수량500 mL
유통 기한12 Months
배송 조건Room Temperature
분류Animal Origin-free
Culture TypeMammalian Cell Culture
형태Liquid
멸균Sterile-filtered
첨가제 포함Glutamine, Phenol Red, Coenzymes
첨가제 없음No HEPES
Unit SizeEach
구성 및 보관
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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