MEM, NEAA, powder
MEM, NEAA, powder
Gibco™

MEM, NEAA, powder

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used자세히 알아보기
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카탈로그 번호수량
4150008350 L
4150003410 x 1 L
4150001810 L
카탈로그 번호 41500083
제품 가격(KRW)
228,000
Each
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수량:
50 L
Customize this product
제품 가격(KRW)
228,000
Each
카트에 추가하기
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With Without
• NEAA • HEPES
• L-glutamine • Sodium Bicarbonate
• Phenol Red

The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco™ MEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ MEM product made in our Scotland facility (41500-067). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

This product is not used for in vitro diagnostic purpose in some countries.
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
사양
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
수량50 L
배송 조건Room Temperature
형태Powder
Serum LevelStandard Serum Supplementation
첨가제 포함Low Glucose, Glutamine, Phenol Red
첨가제 없음No HEPES, No Sodium Pyruvate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

자주 묻는 질문(FAQ)

Do you offer an alternative for the discontinued EMEM (Cat. No. 670086)?

Unfortunately, we do not have a direct replacement for EMEM (Cat. No. 670086). The best alternative is to use powder MEM with NEAA (Cat. No. 41500018) and then add bicarbonate to 1.5 g/L and pyruvate to 1 mM final. This will almost be an exact replacement, but relies on having to make and filter a liquid from the powder.

The next best alternative is to use liquid MEM with NEAA (Cat. No. 10370021). This would require the addition of glutamine (2 mM final) and pyruvate (1 mM final) to equal the concentrations in the discontinued product. In this version, the bicarbonate concentration would be higher (2.2 g/L) than that in the discontinued product (1.5 g/L).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
In vitro biocompatibility of various polymer-based microelectrode arrays for retinal prosthesis.
Authors:Bae SH, Che JH, Seo JM, Jeong J, Kim ET, Lee SW, Koo KI, Suaning GJ, Lovell NH, Cho DI, Kim SJ, Chung H
Journal:Invest Ophthalmol Vis Sci
PubMed ID:22427592
The purpose of our study is to evaluate the biocompatibility of various polymers used as microelectrode arrays (MEAs) in retinal prostheses through in vitro cytotoxicity testing following a standardized ... More