MultiScribe™ Reverse Transcriptase
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MultiScribe™ Reverse Transcriptase
인용 및 참조 문헌 (1)
Invitrogen™

MultiScribe™ Reverse Transcriptase

Applied Biosystems® MultiScribe™ Reverse Transcriptase는 재조합 Moloney murine leukemia virus (rMoMuLV) 역전사효소입니다. MultiScribe™ Reverse Transcriptase는 MuLV reverse transcriptase와 유사하나 권장자세히 알아보기
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카탈로그 번호수량
4311235100 μL
카탈로그 번호 4311235
제품 가격(KRW)
160,000
Online offer
Ends: 31-Dec-2025
177,000
할인액 17,000 (10%)
Each
카트에 추가하기
수량:
100 μL
제품 가격(KRW)
160,000
Online offer
Ends: 31-Dec-2025
177,000
할인액 17,000 (10%)
Each
카트에 추가하기
Applied Biosystems® MultiScribe™ Reverse Transcriptase는 재조합 Moloney murine leukemia virus (rMoMuLV) 역전사효소입니다. MultiScribe™ Reverse Transcriptase는 MuLV reverse transcriptase와 유사하나 권장 용도가 다릅니다. MultiScribe™ Reverse Transcriptase는 보상적 DNA (cDNA) strand 합성 primer가 있는 상태에서 template로 single-stranded RNA를 사용하는 재조합 RNA 의존적 DNA polymerase입니다. 100 μl에 5000 U 제공(50U⁄μl). • 1단계 및 2단계 RT-PCR 반응에 호환되어 분석 설정에 융통성 제공 • 개별 성분 설계로 분석 설정에 활용도가 높습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
최종 제품 유형First-Strand cDNA
형식Stand-alone Enzyme
최적 반응 온도37°C
수량100 μL
반응 형식Separate components
시약 유형Reverse Transcription
역전사 효소MultiScribe
리보뉴클레아제 H 활성Yes
배송 조건Dry Ice
크기(최종 제품)Up to 7 kb
시작 물질RNA
기술Reverse Transcription
농도50 U/μL
용도(애플리케이션)RT-PCR
Unit SizeEach
구성 및 보관

• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)

Store at –20°C.

자주 묻는 질문(FAQ)

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

Can I purchase the MultiScribe Reverse Transcriptase from the TaqMan® MicroRNA Reverse Transcription Kit (Cat. No 4366596) separately?

Yes, the MultiScribe Reverse Transcriptase (Cat. No. 4311235) can be purchased as a standalone product. Please see the following link.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Does Applied Biosystems MultiScribe Reverse Transcriptase have RNase H activity?

Yes, MultiScribe Reverse Transcriptase (Cat. No. 4311235) does have RNase H activity.

What is the difference between MultiScribe Reverse Transcriptase and MuLV Reverse Transcriptase?

MultiScribe Reverse Transcriptase (Cat. No. 4311235) and MuLV Reverse Transcriptase (Cat. No. N808-0018) are the same enzyme, a recombinant Moloney Murine Leukemia Virus (MuLV) Reverse Transcriptase. The only difference is in the recommended usage for each enzyme. MultiScribe Reverse Transcriptase is recommended for use with quantitative nuclease assays, and MuLV is recommended for use in traditional RT-PCR assays.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
PAMP induced expression of immune relevant genes in head kidney leukocytes of rainbow trout (Oncorhynchus mykiss).
Authors:Chettri JK, Raida MK, Holten-Andersen L, Kania PW, Buchmann K
Journal:Dev Comp Immunol
PubMed ID:21147161
'Host immune responses elicited by invading pathogens depend on recognition of the pathogen by specific receptors present on phagocytic cells. However, the reactions to viral, bacterial, parasitic and fungal pathogens vary according to the pathogen-associated molecular patterns (PAMPs) on the surface of the invader. Phagocytic cells are known to initiate ... More