BigDye™ Terminator v1.1 Cycle Sequencing Kit
BigDye™ Terminator v1.1 Cycle Sequencing Kit
Applied Biosystems™

BigDye™ Terminator v1.1 Cycle Sequencing Kit

BigDye™ Terminator v1.1 Cycle Sequencing Kit는 primer에 인접한 최적 basecalling이 필요한 특수 어플리케이션과 신속한 전기영동 작동 모듈의 short PCR product자세히 알아보기
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카탈로그 번호수량
433744924 Reactions
4337450100 Reactions
43374511000 Reactions
43374525000 Reactions
카탈로그 번호 4337449
제품 가격(KRW)
668,000
Each
카트에 추가하기
수량:
24 Reactions
제품 가격(KRW)
668,000
Each
카트에 추가하기
BigDye™ Terminator v1.1 Cycle Sequencing Kit는 primer에 인접한 최적 basecalling이 필요한 특수 어플리케이션과 신속한 전기영동 작동 모듈의 short PCR product templates sequencing을 위해 만들어졌습니다.

• 다양한 sequencing 어플리케이션에 해당하는 결과의 품질 향상.
• 어려운 templates sequence를 성공적으로 수행할 수 있음.
• 보다 일관된 peak와 최적의 signal balance로 보다 오래, 높은 수준의 판독이 가능함.
• 생산성 향상, 비용 절감.

어려운 Sequence 해결
BigDye Terminator v1.1 Cycle Sequencing Kit로 de novo sequencing, resequencing 등 다양한 어플리케이션에 대해 신뢰도와 활용도가 높은 결과를 얻을 수 있습니다.

Peak Height 균일성 및 정확성의 향상
BigDye Terminator v1.1 kit의 우수한 화학 기법으로 균일한 피크 높이와 최적화된 신호 발란스(signal balance) 데이터 및 오랫동안 높은 품질로 유지되는 결과를 얻을 수 있습니다. heterozygote과 mutation 검출을 위한 base assignment 정확성도 높아집니다.

여러분의 업무에 적용이 용이함
BigDye™ Terminator v.1.1 kit 에는 새로운 소프트웨어나 기기의 재보정이 필요하지 않습니다. 최소한의 프로토콜 변경으로 기존 작업 과정에 키트를 쉽게 통합시킬 수 있습니다.

이 제품은 연구용으로만 사용가능합니다. 진단 절차에 사용할 수 없습니다.

For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), Short PCR Product Sequencing, GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, De Novo Sequencing - Low to Mid Throughput
용도(장비)SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐Well and 384‐Well Thermal Cyclers
제품라인BigDye™ Terminator
제품 유형Sequencing Buffer
수량24 Reactions
기술Fluorescent Dye Terminator Sequencing
템플릿 호환성BAC DNA, Plasmid DNA (≤15 Kb), PCR Amplicons, Single Stranded DNA
Unit SizeEach
구성 및 보관
• 200 μL Tube of BigDye™ Terminator v1.1 Ready Reaction Mix
• Tube M13 (-21) Primer
• Tube pREF-BDT™ Control DNA (200 ng/μL)
• 1 mL Tube of 5x Sequencing Buffer (may be stored at 4°C)

Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.

자주 묻는 질문(FAQ)

My DNA sequencing data from the capillary electrophoresis system does not look good. Why?

Capillary electrophoresis systems are complex systems, and troubleshooting data quality can be complicated. The biggest contributors to data quality can be divided into these areas: software, chemistry, and instrument performance.

Troubleshooting software
Ensure that the run module you are using and the dye set match the chemistry and instrument setup. For example, if you are running a multicapillary instrument with a 50 cm array on it, the run module should have a “50” in the name. On a multicapillary system, BigDye Terminator v3.1 should run under Dye Set Z, BigDye Terminator v1.1 chemistry should run in Dye Set E (on the ABI PRISM 310 Genetic Analyzer, both chemistries run under Filter Set E, but require different matrices for analysis). The mobility file (DyeSet/Primer) should have the correct instrument, polymer, and chemistry in the name (e.g. KB_3130_POP7_BDTv.3.mob).

Troubleshooting chemistry and instrument performance
•Sequencing Standards: validate instrument
If Sequencing Standards fail, it suggests a possible electrophoresis problem due to array, polymer, buffer, water, septa, plastics, or maybe a hardware failure such as autosampler, laser, camera, etc.

•pREF-BDT Control DNA: validate cycle sequencing and its clean-up
If the pREF-BDT control reactions fail and the Sequencing Standards pass, look into potential problems with the sequencing kit, thermal cycler, and cleanup procedure.
The pREF-BDT Control DNA and M13 (–21) primers needed to run the reactions are included with each BigDye Terminator Cycle Sequencing Kit

•Custom Internal Controls: validate PCR and its clean-up
If the Sequencing Standards and the pGEM controls passed, then look into potential problems with template quantity and quality, primers, PCR reaction and purification.

For more information on troubleshooting your data, please refer to the DNA Sequencing by Capillary Electrophoresis: Applied Biosystems Chemistry Guide: 3rd Edition, which can be downloaded from this link (https://www.thermofisher.com/us/en/home/global/forms/sanger-sequencing-guide-download-form.html).

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

What are the concentrations of the pREF-BDT and M13 primer controls that come with the BigDye Terminator kits?

The concentration of the pREF-BDT control is 200 ng/µL. The concentration of the M13 primer is 0.8 pmol/µL.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

How do I determine the annealing temperature for the DNA sequencing thermal cycling conditions?



Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

Can I purchase the -21 M13 forward control primer from the BigDye Terminator kit separately?

We do not sell the -21 M13 forward control primer separately, however we provide the primer sequence for your convenience: TGTAAAACGACGGCCAGT

Please feel free to use our oligo services to order this primer (https://www.thermofisher.com/us/en/home/products-and-services/product-types/primers-oligos-nucleotides/invitrogen-custom-dna-oligos.html).
We also offer an -21M13 forward primer that is one basepair shorter than that contained in the kit (Cat. No. N52002).

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

Can I purchase the pREF-BDT control from the BigDye Terminator kit separately?

We do not sell the pREF-BDT control separately from the DNA sequencing kit.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.