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There is no crosslinker as the dyes themselves are made to react directly with the streptavidin protein.Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
There are about 3.993 x 10^16 molecules of dye and 1.141 x 10^16 of streptavidin in 1 mg of Streptavidin-Cy5.Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The target dye to protein ratio for Streptavidin-Cy5 (Cat. No. 434316) is 4:1. The average number of dye molecules on each streptavidin molecule is between 3 and 4.Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.