MicroSEQ™ 500 16S rDNA Sequencing Kit
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인용 및 참조 문헌 (159)
Applied Biosystems™

MicroSEQ™ 500 16S rDNA Sequencing Kit

MicroSEQ™ 500 16S rDNA Sequencing Kit는 MicroSEQ™ 500 16S rDNA Bacterial Identification System의 시퀀싱 구성품으로 DNA sequence에 기반해 쉽게 대부분의자세히 알아보기
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카탈로그 번호수량
43464801 Kit
카탈로그 번호 4346480
제품 가격(KRW)
808,000
Each
카트에 추가하기
수량:
1 Kit
제품 가격(KRW)
808,000
Each
카트에 추가하기
MicroSEQ™ 500 16S rDNA Sequencing Kit는 MicroSEQ™ 500 16S rDNA Bacterial Identification System의 시퀀싱 구성품으로 DNA sequence에 기반해 쉽게 대부분의 박테리아를 확인할 수 있습니다. 이 kit를 사용해 MicroSEQ™ 500 16S rDNA PCR Kit로 생성된 PCR 산물을 시퀀스합니다. 이 두 kit를 함께 사용해 16S rRNA 유전자의 500개 염기쌍을 빠르고 편리하게 증폭할 수 있습니다. 그리고 시퀀스를 MicroSEQ™ 16S rDNA 500 Library와 비교해 일치하는 부분을 생성합니다. 이 kit에는 프로토콜이나 신속 참조 카드가 들어 있지 않습니다.

• Sequence 기반 측정은 24시간 내에 재현성 높은 결과를 보장합니다.
• 성장 요건에 영향을 받지 않는 측정을 통해 객관적인 기준으로 박테리아를 확인할 수 있습니다.
• 보편적인 영역의 primer로 미리 최적화할 필요가 없습니다.
• MicroSEQ™ ID Analysis Software가 정확한 종 확인을 보장합니다.
• 맞춤화된 라이브러리로 새로운 라이브러리를 생성하고 많이 나타나는 미생물이 포함되어 있는 검증된 라이브러리를 검색할 수 있습니다.
• MicroSEQ™ D2 LSU fungal kit와 작업과정이 동일하여 최소한의 교육만 필요합니다.

MicroSEQ™ ID System: 완전한 통합 솔루션
미생물 확인의 모든 단계를 간편화한 Applied Biosystems™ MicroSEQ™ System은 MicroSEQ™ ID Software의 혜택과 쉬운 PrepMan Ultra 샘플 준비 시약과 프로토콜, MicroSeq 박테리아 및 진균 어플리케이션 키트, 업계 최고의 thermal cycler, sequencing 시스템을 모두 통합합니다. 최적의 성능과 시스템 통합을 보장하기 위해 시스템 모든 구성품을 모두 개발하고 검사합니다.

이 제품은 연구용으로만 사용가능합니다. 진단 절차에 사용할 수 없습니다.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Capillary Electrophoresis
용도(애플리케이션)Sequencing
용도(장비)310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer
포함Forward sequencing mix (2 tubes), and reverse sequencing mix (2 tubes)
라이브러리Customizable, Bacterial Library (> 2000 species)
반응 수55 Forward, 55 Reverse
제품라인MicroSEQ
제품 유형Sequencing Kit
수량1 Kit
샘플 종류PCR Products
배송 조건Dry Ice
소프트웨어MicroSEQ™ ID Analysis Software
타겟1st 500 bp of 16S rRNA gene
타겟 유기체 분류Bacteria
기술SBT (Sequence-Based Typing)
워크플로우 단계Cycle Sequencing
형식Kit
Unit SizeEach
구성 및 보관
Each kit contains:
Two tubes each of forward and reverse sequencing mix. Sufficient reagent to perform 55 forward and 55 reverse sequencing reactions. Store at -15 to -25°C.

자주 묻는 질문(FAQ)

Which thermocyclers are compatible with the MicroSEQ 500 16S rDNA Sequencing Kit?

To take advantage of the reduced amplification and sequencing times allowed by the new fast PCR chemistry and the 9800 Fast Thermal Cycler, we recommend using the Applied Biosystems 9800 Fast Thermal Cycler with the MicroSEQ kits. The GeneAmp PCR System 9700 may also be used but note that the 9700 thermocycler does not provide the reduced amplification and sequencing times allowed by the new fast PCR chemistry and the 9800 Fast Thermal Cycler.

Do you have stability information for the MicroSEQ 500 16S rDNA Sequencing Kit (Cat. No. 4346480) when subjected to temperature fluctuations?

The MicroSEQ 500 16S rDNA Sequencing Kit is stable if stored at -20 degrees C for 15-16 months. We do not have any information available for temperatures outside of this range.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

Is the MicroSEQ 500 16S rDNA Sequencing Kit (Cat. No. 4346480) FDA approved?

No, it is for RUO.

인용 및 참조 문헌 (159)

인용 및 참조 문헌
Abstract
Structures of aldouronic acids liberated from kenaf xylan by endoxylanases from Streptomyces sp.
Authors:Komiyama, H; Enomoto, A; Sueyoshi, Y; Nishio, T; Kato, A; Ishii, T; Shimizu, K
Journal:
PubMed ID:
'Streptomyces sp. SK519 was cultivated in the medium containing 0.5% birch wood xylan to produce extracellular xylanase. The xylanase was isolated from the culture supernatant by ammonium sulfate precipitation and partially purified by absorbing on the insoluble xylan. Alkali-extracted kenaf glucuronoylan of which main chain primarily consisting of (1-4)-linked β-D-xylopyranose ... More
Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site
Authors:Gupta, SK; Kumari, R; Prakash, O; Lal, R
Journal:
PubMed ID:
'A Gram-negative, motile, rod-shaped, non-sporulating, aerobic bacterial strain (Esp-1(T)) was isolated from oil-contaminated soil of Panipat Oil Refinery, India, and its taxonomic position was determined using a polyphasic approach. Strain Esp-1(T) grew in the presence of 2% NaCl at 30 °C and was characterized chemotaxonomically by having C(16:0) as the ... More
Multilocus DNA sequence comparisons rapidly identify pathogenic molds.
Authors:Rakeman JL; Bui U; Lafe K; Chen YC; Honeycutt RJ; Cookson BT
Journal:
PubMed ID:
'The increasing incidence of opportunistic fungal infections necessitates rapid and accurate identification of the associated fungi to facilitate optimal patient treatment. Traditional phenotype-based identification methods utilized in clinical laboratories rely on the production and recognition of reproductive structures, making identification difficult or impossible when these structures are not observed. We ... More
Species distribution and antimicrobial resistance of enterococci isolated from surface and ocean water
Authors:Moore, DF; Guzman, JA; Mcgee, C
Journal:
PubMed ID:
'Aims: The species identification and antimicrobial resistance profiles were determined for enterococci isolated from Southern California surface and ocean waters. Methods and Results: Species identification was determined for 1413 presumptive Enterococcus isolates from urban runoff, bay, ocean and sewage water samples. The most frequently isolated species were Enterococcus faecalis, Enterococcus ... More
Multicenter evaluation of the new VITEK 2 advanced colorimetric yeast identification card.
Authors:Hata DJ; Hall L; Fothergill AW; Larone DH; Wengenack NL
Journal:
PubMed ID:
'The performance of the new VITEK 2 Advanced Colorimetry yeast identification (YST) card for use with the VITEK 2 system (bioMerieux, Inc., Hazelwood, MO) was compared to that of the API 20C AUX (API) system (bioMerieux SA, Marcy-l''Etoile, France) in a multicenter evaluation. A total of 12 quality control, 64 ... More
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