MicroSEQ™ 500 16S rDNA PCR Kit
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인용 및 참조 문헌 (159)
Applied Biosystems™

MicroSEQ™ 500 16S rDNA PCR Kit

MicroSEQ™ 500 16S rDNA PCR Kit는 MicroSEQ™ 500 16S rDNA Bacterial Identification System의 PCR 구성품으로 DNA sequence에 기반하여 쉽게 대부분의자세히 알아보기
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카탈로그 번호수량
43482281 kit
카탈로그 번호 4348228
제품 가격(KRW)
1,421,000
Each
카트에 추가하기
수량:
1 kit
제품 가격(KRW)
1,421,000
Each
카트에 추가하기
MicroSEQ™ 500 16S rDNA PCR Kit는 MicroSEQ™ 500 16S rDNA Bacterial Identification System의 PCR 구성품으로 DNA sequence에 기반하여 쉽게 대부분의 박테리아를 확인할 수 있습니다. 이 kit에서 생성된 PCR-증폭 산물은 MicroSEQ™ 500 16S rDNA Sequencing Kit를 사용해 sequencing할 수 있습니다. 이 두 kit를 함께 사용해 16S rRNA 유전자 500쌍 염기를 빠르고 쉽게 증폭하고 sequencing한 후 MicroSEQ™ 16S rDNA 500 Library와 비교해 일치하는 유전자를 확인할 수 있습니다. 이 kit에는 프로토콜이나 신속 참조 카드가 들어있지 않습니다.

• Sequence 기반 측정은 24시간 내에 재현성 높은 결과를 보장합니다.
• 성장 요건에 영향을 받지 않는 측정을 통해 객관적인 기준으로 박테리아를 확인할 수 있습니다.
• 보편적인 영역의 primer로 미리 최적화할 필요가 없습니다.
• MicroSEQ ID Analysis Software가 정확한 종 확인을 보장합니다.
• 맞춤화된 라이브러리로 새로운 라이브러리를 생성하고 많이 나타나는 미생물이 포함되어 있는 검증된 라이브러리를 검색할 수 있습니다.
• MicroSEQ D2 LSU fungal kit와 작업과정이 동일하여 최소한의 교육만 필요합니다.

MicroSEQ™ ID System: 완전한 통합 솔루션
미생물 확인의 모든 단계를 간편화한 Applied Biosystems MicroSeq System은 MicroSEQ ID Software의 혜택과 쉬운 PrepMan Ultra 샘플 준비 시약과 프로토콜, MicroSeq 박테리아 및 진균 어플리케이션 키트, 업계 최고의 thermal cycler, sequencing 시스템을 모두 통합합니다. 최적의 성능과 시스템 통합을 보장하기 위해 시스템 모든 구성품을 모두 개발하고 검사합니다.

이 제품은 연구용으로만 사용가능합니다. 진단 절차에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)GeneAmp 9700, Veriti Thermal Cycler
라이브러리Customizable, Bacterial Library (> 2000 species)
제품라인MicroSEQ
제품 유형PCR Kit
수량1 kit
샘플 종류DNA (Genomic)
배송 조건Dry Ice
소프트웨어MicroSEQ™ ID Analysis Software
타겟1st 500 bp of 16S rRNA gene
타겟 유기체 분류Bacteria
기술PCR Amplification, SBT (Sequence-Based Typing)
검출 방법Primer-probe
용도(애플리케이션)Microbial Detection
Unit SizeEach
구성 및 보관
Each kit contains enough PCR Master Mix to perform 50 PCR amplifications, 5 negative control assays, and 5 positive control assays. One tube of positive control E. coli DNA, and one of water (for use as a negative control) are also included. Store at -15 to -25°C upon reciept. After first use, store at 2–8 °C in a PCR Clean Room.

자주 묻는 질문(FAQ)

What is the sensitivity of the MicroSeq 500 kits?

As long as you rstart from a visible colony or cell pellet, MicroSeq kits will work.

인용 및 참조 문헌 (159)

인용 및 참조 문헌
Abstract
Structures of aldouronic acids liberated from kenaf xylan by endoxylanases from Streptomyces sp.
Authors:Komiyama, H; Enomoto, A; Sueyoshi, Y; Nishio, T; Kato, A; Ishii, T; Shimizu, K
Journal:
PubMed ID:
'Streptomyces sp. SK519 was cultivated in the medium containing 0.5% birch wood xylan to produce extracellular xylanase. The xylanase was isolated from the culture supernatant by ammonium sulfate precipitation and partially purified by absorbing on the insoluble xylan. Alkali-extracted kenaf glucuronoylan of which main chain primarily consisting of (1-4)-linked β-D-xylopyranose ... More
Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site
Authors:Gupta, SK; Kumari, R; Prakash, O; Lal, R
Journal:
PubMed ID:
'A Gram-negative, motile, rod-shaped, non-sporulating, aerobic bacterial strain (Esp-1(T)) was isolated from oil-contaminated soil of Panipat Oil Refinery, India, and its taxonomic position was determined using a polyphasic approach. Strain Esp-1(T) grew in the presence of 2% NaCl at 30 °C and was characterized chemotaxonomically by having C(16:0) as the ... More
Multilocus DNA sequence comparisons rapidly identify pathogenic molds.
Authors:Rakeman JL; Bui U; Lafe K; Chen YC; Honeycutt RJ; Cookson BT
Journal:
PubMed ID:
'The increasing incidence of opportunistic fungal infections necessitates rapid and accurate identification of the associated fungi to facilitate optimal patient treatment. Traditional phenotype-based identification methods utilized in clinical laboratories rely on the production and recognition of reproductive structures, making identification difficult or impossible when these structures are not observed. We ... More
Species distribution and antimicrobial resistance of enterococci isolated from surface and ocean water
Authors:Moore, DF; Guzman, JA; Mcgee, C
Journal:
PubMed ID:
'Aims: The species identification and antimicrobial resistance profiles were determined for enterococci isolated from Southern California surface and ocean waters. Methods and Results: Species identification was determined for 1413 presumptive Enterococcus isolates from urban runoff, bay, ocean and sewage water samples. The most frequently isolated species were Enterococcus faecalis, Enterococcus ... More
Multicenter evaluation of the new VITEK 2 advanced colorimetric yeast identification card.
Authors:Hata DJ; Hall L; Fothergill AW; Larone DH; Wengenack NL
Journal:
PubMed ID:
'The performance of the new VITEK 2 Advanced Colorimetry yeast identification (YST) card for use with the VITEK 2 system (bioMerieux, Inc., Hazelwood, MO) was compared to that of the API 20C AUX (API) system (bioMerieux SA, Marcy-l''Etoile, France) in a multicenter evaluation. A total of 12 quality control, 64 ... More
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