7500 Real-Time PCR System, laptop

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The discontinuation of sales for the 7500 and 7500 Fast real-time PCR for RUO starts on October 31, 2025. The 7500 Fast Dx was discontinued on December 31, 2022. Visit QuantStudio Real-Time PCR Systems for your qPCR instrument needs
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인용 및 참조 문헌 (38623)

Applied Biosystems™

7500 Real-Time PCR System, laptop

본 제품은 DNA amplifier 질병 진단을 위해 특정 유전자(DNA)의 증폭에 사용하는 장치입니다. 중합 효소 연쇄반응(Polymerase chain reaction) 과정을 사용하여 핵산을자세히 알아보기

카탈로그 번호	수량
4351104	1 system

카탈로그 번호 4351104

제품 가격(KRW)

견적 요청하기

1 system

본 제품은 DNA amplifier 질병 진단을 위해 특정 유전자(DNA)의 증폭에 사용하는 장치입니다. 중합 효소 연쇄반응(Polymerase chain reaction) 과정을 사용하여 핵산을 자동적으로 증폭하고 실시간으로 PCR 증폭 산물의 생성과정을 모니터링하여 target DNA의 양을 분석합니다.

For Research Use Only. Not for use in diagnostic procedures.

보정된 염료SYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™

치수Width 34 cm (13.39 in.), Depth 45 cm (17.72 in.), Height 49 cm (19.29 in.)

동적 범위9 Logs

전기 요구사항950 W

외부 컴퓨터Notebook: Intel Core 2 Duo T5500 (1.66 GHz), 8X DVD, 80 GB HD, 1.0 G DDR2-533 MHz SDRAM

용도(장비)7500 System

형식96-well Plate

고처리량 호환성Multiplexing

최대 가열 속도(열 블록)2.5°C

옵틱스CCD Camera, 5 Excitation Filters, Tungsten-halogen Lamp, 5 Emission Filters

패시브 참조 염료ROX (Separate Tube), No ROX, ROX (Pre-mixed)

피크 블록 램프 속도2.5°C⁄s

정밀도99.7% Confidence (5000 & 10000 Copies)

수량1 system

반응 속도Standard

반응 부피 범위20–100 μL

샘플 램프 속도± 1.1°C⁄s

민감도1 Copy of RNase P gene (Human Genomic DNA)

소프트웨어 호환성Primer Express, 21 CFR Part 11 Module (optional), 7500 System Software (RQ included)

온도 범위(미터법)4 to 100°C

열 정확도0.25°C (35°C to 95°C after 3 min.)

열 사이클링 시스템Peltier-based System

열 균일성0.50°C (after 30 s)

처리량4 to 5-96-well Plates per 8-hr day

전압120 V

무게34 kg (75 lb.)

Unit SizeEach

구성 및 보관

Notebook Computer

자주 묻는 질문(FAQ)

Why are there no RQ values for all the samples in my study from the 7500 or 7500 Fast Real-Time PCR System software?

The 7500 software requires that the endogenous control be run on every plate within a study. If not, you will only see RQ values for the plate that contains the endogenous control on it. The other plates will have CT values for all the wells, but the software will not calculate RQ values.

If you designed your experiments in this type of layout, you will want to do your analysis in the free DataAssist software instead. This program does not have the endogenous control requirement for every plate. Simply export the study results file from the 7500 software as a *.txt or *.csv file, and use that to create a new study in the DataAssist software. You should now be able to see RQ values for all samples, even those without an endogenous control on the same plate.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why do I not see any CT or RQ values from my ddCT experiment in the software for the 7500 or 7500 Fast Real-Time PCR System?

If you have the SDS v1.4 or earlier, you will need to open the dd CT plate in a Study first in order to see the CT or RQ values. (If you have v2.0.x this error does not apply.)

1.After the initial run is complete, open, review the plots and save it. Then:
-Choose File then New
-Choose: Assay then ddCT (Relative Quantitation) Study. Set the other parameters as appropriate and click Next.
2.Click on ‘Add Plates...', then browse to where your data file is located and select the plate (or multiple plates).
Click ‘Finish', and you should see your study.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What can I do when I get the error message "Time too short" on the 7500 or 7500 Fast Real-Time PCR System?

You may encounter this error message, which will abort the run before it even starts. If so, go into the software under Setup then Run Method and check how much time has been set for the data collection step. The instrument has a minimum collection time, which is dependent on the number of filters being used. (This is true in both the 1.x and 2.0.x versions of the 7500 software.) Increase the extension time to 30 sec and restart the run. It should now proceed as normal. If the error persists, please contact us at techsupport@thermofisher.com for further assistance.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What can I do if I get an error message about "Block Up Switch" on the 7500 or 7500 Fast Real-Time PCR System?

This error can be caused by the wrong plastics being used in the block, such as the wrong Precision Plate Holder being used in the tray. This can give an error message such as “Block up switch not activating” and will not allow the run to start. Open the drawer, make sure the correct plate/tubes and plate/tube holder are being used and in the correct orientation. Turn off the instrument and open the front cover to make sure the heated cover door is closed. Restart the instrument and retry the run. If the error still persists, please contact us at instrumentservices@thermofisher.com for further assistance.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

On the 7500 and 7500 Fast Real-Time PCR Systems, what can I do if I get an error message about "CCD acquisition failure"?

If you see this error message, please check the following:

1. Verify that the USB connection is secure between the instrument and computer.
2. Verify that the computer user power save settings are set to Never for all users (in Windows under Control Panel, Power Options).
3. Is there a screen saver in use? If so, set the screen saver so that it will not come on during a run.
4. Is there antivirus or other network software in use? If so, disconnect from the network while running the instrument.
If the error persists, please contact us at instrumentservices@thermofisher.com for further assistance.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

View all 7500 Real-Time PCR System, laptop FAQs

인용 및 참조 문헌 (38623)

인용 및 참조 문헌

Abstract

Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress: Use of APE1 small molecule inhibitors to delineate APE1 functions

Authors:Jiang, YL; Guo, CL; Fishel, ML; Wang, ZY; Vasko, MR; Kelley, MR

Journal:DNA REPAIR

PubMed ID:

Oxidative DNA damage has been implicated in a number of central nervous system pathologies. The base excision repair (BER) pathway is one of the most important cellular protection mechanisms that respond to oxidative DNA damage. Human apurinic (apyrimidinic) endonuclease/redox effector factor (APE1/Ref1 or APE1) is an essential enzyme in the ... More

Characterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 and their inhibitors in equine granulosa cells in vivo and in vitro

Authors:Sessions, DR; Vick, MM; Fitzgerald, BP

Journal:JOURNAL OF ANIMAL SCIENCE

PubMed ID:

Matrix metalloproteinases (MMP) and tissue inhibitors of MMP (TIMP) regulate tissue remodeling events necessary for ovulation. Thus, changes in MMP and TIMP expression and protein enzyme activity were examined in vivo and in vitro during follicular development and atresia in the horse. Equine granulosa cells and follicular fluid from medium ... More

The time of prenatal immune challenge determines the specificity of inflammation-mediated brain and behavioral pathology.

Authors:Meyer U, Nyffeler M, Engler A, Urwyler A, Schedlowski M, Knuesel I, Yee BK, Feldon J

Journal:J Neurosci

PubMed ID:16672647

Disturbance to early brain development is implicated in several neuropsychiatric disorders including autism, schizophrenia, and mental retardation. Epidemiological studies have indicated that the risk of developing these disorders is enhanced by prenatal maternal infection, presumably as a result of neurodevelopmental defects triggered by cytokine-related inflammatory events. Here, we demonstrate that ... More

Distinct roles for IFN regulatory factor (IRF)-3 and IRF-7 in the activation of antitumor properties of human macrophages.

Authors:Romieu-Mourez R, Solis M, Nardin A, Goubau D, Baron-Bodo V, Lin R, Massie B, Salcedo M, Hiscott J

Journal:Cancer Res

PubMed ID:17079482

When properly activated, macrophages can be tumoricidal, thus making them attractive additions to standard cancer therapies. To this end, tolerance and activity of human autologous IFN-gamma-activated macrophages, produced in large scale for clinical use (MAK cells), have been assessed in pilot trials in cancer patients. In the present study, we ... More

Trps1 plays a pivotal role downstream of Gdf5 signaling in promoting chondrogenesis and apoptosis of ATDC5 cells.

Authors:Itoh S, Kanno S, Gai Z, Suemoto H, Kawakatsu M, Tanishima H, Morimoto Y, Nishioka K, Hatamura I, Yoshida M, Muragaki Y

Journal:Genes Cells

PubMed ID:18363966

Tricho-rhino-phalangeal syndrome (TRPS) is an autosomal dominant skeletal disorder caused by mutations of TRPS1. Based on the similar expression patterns of Trps1 and Gdf5, we hypothesized a possible functional interaction between these two molecules. Using a chondrogenic cell line (ATDC5), we investigated the association of Gdf5-mediated signaling pathways with ... More

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