TaqMan™ SNP Genotyping Assay, human
TaqMan™ SNP Genotyping Assay, human
인용 및 참조 문헌 (1421)
Applied Biosystems™

TaqMan™ SNP Genotyping Assay, human

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Applied Biosystems TaqMan SNP Genotyping Assay는 정제된 게놈 DNA 샘플에서 특정 다형성 유전자를 증폭 및 검출하기 위해 TaqMan 5´‑nuclease 화학자세히 알아보기
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카탈로그 번호수량
43513742400회 반응(대형, 80X)
43513761000회 반응(중형, 40X)
4351379300회 반응(소형, 40X)
카탈로그 번호 4351374
제품 가격(KRW)
2,029,000
Precio exclusivo en nuestra web
Ends: 31-Dec-2025
2,254,000
할인액 225,000 (10%)
Each
주문하기
수량:
2400회 반응(대형, 80X)
Applied Biosystems TaqMan SNP Genotyping Assay는 정제된 게놈 DNA 샘플에서 특정 다형성 유전자를 증폭 및 검출하기 위해 TaqMan 5´‑nuclease 화학 기술을 사용합니다. 각 분석은 단일 뉴클레오티드 다형성(SNP)에 대한 개별 유전형 분석을 가능하게 하며, 2개의 염기서열 특이적 프라이머 및 무형광 NFQ를 갖는 2개의 TaqMan MGB(minor groove binder) 프로브로 구성됩니다. 한 프로브에는 대립 유전자 1 시퀀스를 검출하기 위해 VIC 염료가 라벨링되어 있습니다. 두 번째 프로브에는 대립유전자 2 서열을 검출하기 위해 FAM 염료를 라벨링합니다.

당사의 사전 설계된 TaqMan SNP 유전형 분석 인간 어세이는 전 게놈 범위에서 일반적인 1,000 게놈 SNP, HapMap SNP 및 코딩 SNP를 비롯한 수백만 개의 인간 어세이를 제공합니다.

장점:
• 검증됨— gold-standard TaqMan 화학 및 강력한 분석 설계는 정확하고 재현성 있고 신뢰할 수 있는 결과를 제공합니다.
• 간편함— 단일 튜브 형식과 간단한 워크플로우로 신뢰할 수 있는 결과를 쉽게 얻을 수 있어 최적화가 필요하지 않습니다.
• 관련성— 사전 설계된 인간 분석의 광범위한 수집을 통해 연구와 관련된 콘텐츠에 직접 액세스할 수 있습니다.
• 테스트됨— 모든 인간 SNP 유전자형 검사는 모든 유전자 변별을 확인하기 위해 기능적으로 테스트됩니다.

대략적인 배송 시간
북미의 경우 4–6일, 유럽의 경우 6–10일

TaqMan SNP Genotyping Assay는 PCR을 위한 3가지 반응 구성요소, 즉 정제된 게놈 DNA(1–20ng), 분석 용액, TaqMan Genotyping Master Mix(또는 다른 호환 가능한 마스터 믹스)(별도 판매)만 필요합니다.

모든 분석 설계는 제조 및 분석 성능 데이터를 활용하여 10년 넘게 최적화된 당사의 생물정보학 파이프라인의 산물입니다. TaqMan 어세이는 40,000 이상의 논문에 인용되었으며 350개 이상의 특허에 의해 뒷받침되고 있습니다.

당사의 사전 설계된 모든 TaqMan 어세이는 TaqMan 어세이 개런티로 적용됩니다. *

권장되는 마스터 믹스(별도 판매): TaqMan Genotyping Master Mix

* 이용 약관이 적용됩니다. 자세한 내용은 www.thermofisher.com/taqmanguarantee 참조하십시오.

For Research Use Only. Not for use in diagnostic procedures.
사양
3'프라이머 수정없음
5'프라이머 수정없음
설명VIC™ - MGB probe detects Allele 1, FAM - MGB probe detects Allele 2
용도(장비)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ Absolute Q Digital PCR System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals.
그린 기능환경친화적인 포장
내부 프로브 수정VIC™(5'), FAM(5'), MGB(Minor Groove Binder)(3')
반응 수2400 reactions
제품라인TaqMan
정제 방법고체상 추출법(SPE)
순도 또는 품질 등급RNase 비함유, DNase 비함유
수량2400회 반응(대형, 80X)
샘플 종류Human
배송 조건실온
종Human
타겟Over 4.5 million genome-wide human assays are available
농도80X
용도(애플리케이션)Genotyping
형태냉동 상태
라벨 또는 염료FAM, VIC
Unit SizeEach
구성 및 보관
40X (S 및 M 크기) 또는 80X (L 크기)의 사전 조제된 어세이 믹스가 포함된 튜브 1개(프로브 2개 및 프라이머 2개).

-15 ∼ -25°C에서 보관.

자주 묻는 질문(FAQ)

How do I set up a reference panel in the TaqMan Genotyper Software?

A reference panel is helpful in large studies to mark your reference samples. Please follow the directions here on how to set up a reference panel.

How do I enter the polymorphism sequence information (i.e., A, C, G, T) for my assays, and where is this info displayed in the TaqMan Genotyper Software?

The polymorphism sequence info can be entered into the software through Setup >Assays. You can import an assay information file (AIF) that contains this info for your assays (AIFs are shipped with assay orders), or manually enter this info for each assay using the edit assay feature. The polymorphism sequence info will be displayed in the assays table under allele1 base and allele2 base, in the results table in the calls column, in the cluster plot display in the x-axis and y-axis titles, and in the export files as genotypes. If no sequence information is entered for an assay, the default display for genotype calls will use the dye names, such as VIC/VIC, VIC/FAM or FAM/FAM dyes.

What is the bookmarking feature in the TaqMan Genotyper Software, and how would I use it?

Bookmarking is a unique feature in TaqMan Genotyper Software that allows you to tag a data point or well while reviewing results in a Study. For example, in reviewing a cluster plot for an assay, a data point is observed to be somewhat between clusters. You can set a bookmark for this data point to denote this well for further investigation. The bookmark persists between the Results workspace and Quality Control workspace, so you can easily identify the data point in a cluster plot, experiment plate view, or on the samples tab. Bookmarks are cleared upon exit from a Study or exit from the application.

I am getting the message: "An error has occurred. See the log file C:\ProgramFiles\Applied Biosystems\TaqMan Genotyper\config\eclipse\1363113099385.log." How can I fix this?

1.Go to the Start button, then Programs, then TaqMan Genotyper Software
2.Right-click on the program and choose “Run as Administrator”
3.If that does not work, go back to the same menu and choose “Properties”
4.Choose the “Compatibility” tab, and check “Run this program as administrator”
5.Click “Apply”
6.You may+C69 have to restart the computer for the settings to apply

Can I delete an assay or sample from my qPCR study?

An assay or sample may be deleted from a study only if there is no data or wells associated with it. Upon import of an experiment, the software collects all the assays and samples from the plate and lists them in the Setup > Assays or Setup > Samples workspaces. The assays and samples are stored in these workspaces as a library, and remain there even if you delete the experiment from the Study. Deleting the experiment will remove any data (wells) associated with the assays or samples, but not the assays or samples from the library. The assays and samples must then be deleted from these workspaces to remove them from the Study.

View all TaqMan™ SNP Genotyping Assay, human FAQs

인용 및 참조 문헌 (1421)

인용 및 참조 문헌
Abstract
Determination, mechanism and monitoring of knockdown resistance in permethrin-resistant human head lice, Pediculus humanus capitis
Authors:Clark, JM
Journal:JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
PubMed ID:
Permethrin resistance has been reported worldwide and clinical failures to commercial pediculicides containing permethrin have likewise occurred. Permethrin resistance in head lice populations from the U.S. is widespread but is not yet uniform and the level of resistance is relatively low (∼4-8 fold). Permethrin-resistant lice are cross-resistant to pyrethrins, PBO-synergized ... More
Geographical mapping of a multifocal thyroid tumour using genetic alteration analysis & miRNA profiling
Authors:Aherne, ST; Smyth, PC; Flavin, RJ; Russell, SM; Denning, KM; Li, JH; Guenther, SM; O'Leary, JJ; Sheils, OM
Journal:Molecular Cancer
PubMed ID:
Background: Papillary thyroid carcinoma (PTC) frequently presents as multiple tumour-foci within a single thyroid gland or pluriform, with synchronous tumours comprising different histological variants, raising questions regarding its clonality. Among the genetic aberrations described in PTC, the BRAF V600E mutation and ret/PTC activation occur most commonly. Several studies have investigated ... More
Risk haplotype analysis for bovine paratuberculosis
Authors:Pinedo, PJ; Wang, CG; Li, Y; Rae, DO; Wu, RL
Journal:MAMMALIAN GENOME
PubMed ID:
Paratuberculosis (Johne's disease), caused by Mycobacterium avium subsp. paratuberculosis, is an important disease for bovines, although its genetic basis is poorly understood. In this study, three candidate genes were typed to study the associations between single nucleotide polymorphisms (SNPs) and paratuberculosis susceptibility (measured in a 1 or 0 form) at ... More
Risk haplotype analysis for bovine paratuberculosis
Authors:Pinedo, PJ; Wang, CG; Li, Y; Rae, DO; Wu, RL
Journal:JOURNAL OF NEURAL TRANSMISSION
PubMed ID:
Paratuberculosis (Johne's disease), caused by Mycobacterium avium subsp. paratuberculosis, is an important disease for bovines, although its genetic basis is poorly understood. In this study, three candidate genes were typed to study the associations between single nucleotide polymorphisms (SNPs) and paratuberculosis susceptibility (measured in a 1 or 0 form) at ... More
Association of Polymorphisms in Genes of the Homologous Recombination DNA Repair Pathway and Thyroid Cancer Risk
Authors:Bastos, HN; Antao, MR; Silva, SN; Azevedo, AP; Manita, I; Teixeira, V; Pina, JE; Gil, OM; Ferreira, TC; Limbert, E; Rueff, J; Gaspar, JF
Journal:THYROID
PubMed ID:
Background: Ionizing radiation exposure has been pointed out as a risk factor for thyroid cancer. The double-strand breaks induced by this carcinogen are usually repaired by homologous recombination repair pathway, a pathway that includes several polymorphic genes. Since there is a scarcity of data about the involvement of these gene ... More
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