TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG
TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG
Actual product may vary
인용 및 참조 문헌 (43)
Applied Biosystems™

TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR in자세히 알아보기
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호반응 수
4352042250 Reactions
4366072500 Reactions
43678465000 Reactions
43660731250 Reactions
43641032500 Reactions
카탈로그 번호 4352042
제품 가격(KRW)
362,000
Online offer
Ends: 31-Dec-2025
517,000
할인액 155,000 (30%)
Each
카트에 추가하기
반응 수:
250 Reactions
제품 가격(KRW)
362,000
Online offer
Ends: 31-Dec-2025
517,000
할인액 155,000 (30%)
Each
카트에 추가하기
TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR in a 20-µL reaction volume using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems. The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments.

Convenience
Everything you need for TaqMan-based qPCR amplification and detection in a single-tube format. TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Compatibility
TaqMan Fast Universal PCR Master Mix (2X), No AmpErase UNG, is compatible with TaqMan Gene Expression assays.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we've taken the best of TaqMan Fast Universal PCR Master Mix and added additional capabilities for your gene expression analysis.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)2 X
용도(장비)7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
형식Tube
반응 수250 Reactions
패시브 참조 염료ROX (Pre-mixed)
중합효소AmpliTaq DNA Polymerase
제품라인AmpErase, TaqMan
제품 유형Fast Universal PCR Master Mix
수량250 reactions
샘플 종류DNA (Genomic), cDNA
배송 조건Wet Ice
충분250 Reactions
부피20 μL
농도2X
검출 방법Primer-probe
용도(애플리케이션)Gene Expression, Genotyping, miRNA analysis
GC-Rich PCR PerformanceHigh
PCR 방법qPCR
반응 속도Fast
Unit SizeEach
구성 및 보관
TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains two tubes of 1.25 mL each sufficient for 250 20-μL PCR reactions. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

자주 묻는 질문(FAQ)

Can SNP assays be run using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

It may be technically feasible, but we have not fully validated TaqMan SNP Genotyping assays on the Applied Biosystems 7500 Fast System and therefore cannot recommend it.

What real-time PCR applications can I perform using the TaqMan Fast Universal PCR Master Mix (Cat. No. 4352042)?

You can perform experiments for Absolute Quantitation and Relative Quantitation with fast cycling. However, Allelic Discrimination (SNP) assays and plus/minus assays using internal positive controls cannot be run with fast reagents and protocols.

인용 및 참조 문헌 (43)

인용 및 참조 문헌
Abstract
Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro.
Authors:Sugiyama K, Suzuki K, Nakazawa T, Funami K, Hishiki T, Ogawa K, Saito S, Shimotohno KW, Suzuki T, Shimizu Y, Tobita R, Hijikata M, Takaku H, Shimotohno K
Journal:J Virol
PubMed ID:19369330
Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5' untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, ... More
Down Regulation of Genes Involved in T Cell Polarity and Motility during the Induction of Heart Allograft Tolerance by Allochimeric MHC I
Authors:Lisik, W; Tejpal, N; Gong, YQ; Skelton, TS; Ganachari, M; Bremer, EG; Kloc, M; Ghobrial, RM
Journal:
PubMed ID:
'Background: The allochimeric MHC class I molecule [α1h1/u]-RT1. Aa that contains donor-type (Wistar Furth, WF; RT1u) epitopes displayed on recipient-type (ACI, RT1a) administered in conjunction with sub-therapeutic dose of cyclosporine (CsA) induces indefinite survival of heterotopic cardiac allografts in rat model. In vascularized transplantation models, the spleen contributes to graft ... More
Activation of Macrophages by Polysaccharide-protein Complex from Lycium barbarum L.
Authors:Chen, ZS; Soo, MY; Srinivasan, N; Tan, BKH; Chan, SH
Journal:
PubMed ID:
'Macrophages play crucial roles in innate immunity. This paper reports that a polysaccharide-protein complex isolated from Lycium barbarum (LBP) is able to activate macrophages. LBP was isolated from Lycium barbarum fruit and separated to five homogenous fractions, designated LBPF1, LBPF2, LBPF3, LBPF4 and LBPF5. It was found that LBP (50 ... More
Fusion of Epstein-Barr virus nuclear antigen-1-derived glycine-alanine repeat to trans-dominant HIV-1 Gag increases inhibitory activities and survival of transduced cells in vivo
Authors:Hammer, D; Wild, J; Ludwig, C; Asbach, B; Notka, F; Wagner, R
Journal:
PubMed ID:
'Trans-dominant human immunodeficiency virus type 1 (HIV-1) Gag derivatives have been shown to efficiently inhibit late steps of HIV-1 replication in vitro by interfering with Gag precursor assembly, thus ranking among the interesting candidates for gene therapy approaches. However, efficient antiviral activities of corresponding transgenes are likely to be counteracted ... More
Prevalence of Chlamydophila psittaci in wild birds-potential risk for domestic poultry, pet birds, and public health?
Authors:Zweifel, D; Hoop, R; Sachse, K; Pospischil, A; Borel, N
Journal:
PubMed ID:
'To determine the prevalence of Chlamydophila psittaci in wild birds, cloacal swabs from 527 songbirds, 442 waterfowl, 84 feral pigeons, and 38 cormorants were examined by Chlamydiaceae-specific real-time polymerase chain reaction (PCR) and ArrayTube microarray assays for chlamydial species determination and genotyping of C. psittaci. Inconclusive cases were further characterized ... More
View all TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG, 250 reactions citations