Plate and Module with Affinity Binding Surface

The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.

High background signal could be due to inadequate washing. Make sure to pre-wash the Nunc Immobilizer Streptavidin Plates/Strips and increase the number of washing steps and the time between washes. Another possible cause is too high a concentration of the detection components.

Our Nunc Immobilizer Streptavidin Plates/Strips are extremely stable over time, if stored at room temperature (20 degrees C to 25 degrees C). If kept in the unopened foil packaging, this product should have an 18 month shelf life after the date of manufacturing.

Coupling of the target molecule to the streptavidin proceeds very rapidly. Results show that incubation with gentle agitation for 2 hr at room temperature is typically sufficient to saturate all available active site.

The total binding capacity for free biotin is:
- 20 pmol/well in a 96 well (coating volume 100 µL)
- 10 pmol/well in a 384 well (coating volume 50 µL)