TaqMan™ MicroRNA Reverse Transcription Kit
TaqMan™ MicroRNA Reverse Transcription Kit
Applied Biosystems™

TaqMan™ MicroRNA Reverse Transcription Kit

Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit는 TaqMan™ MicroRNA Assay에서 최적화된 성능의 반응 200회에 필요한 성분을 제공합니다. 이 키트 구성은자세히 알아보기
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카탈로그 번호충분
43665971000 Reactions
4366596200 Reactions
카탈로그 번호 4366597
제품 가격(KRW)
1,519,000
キャンペーン価格
Ends: 31-Dec-2025
1,687,000
할인액 168,000 (10%)
Each
카트에 추가하기
충분:
1000 Reactions
제품 가격(KRW)
1,519,000
キャンペーン価格
Ends: 31-Dec-2025
1,687,000
할인액 168,000 (10%)
Each
카트에 추가하기
Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit는 TaqMan™ MicroRNA Assay에서 최적화된 성능의 반응 200회에 필요한 성분을 제공합니다. 이 키트 구성은 TaqMan™ MicroRNA Assay와 함께 제공되는 RT primer과 함께 사용하여 miRNA 를 cDNA로 전환합니다.

전구 물질이 아닌 성숙한 miRNA만을 정량하도록 만들어진 매우 구체적인 kit이며, 제한된 샘플을 아껴 씀으로써 총 RNA 1∼10 nanogram만 필요로 하는 민감한 키트입니다. 빠르고 간편하며 확장성 높은 2단계 정량 RT-PCR assay로 3시간 내에 품질 높은 결과를 제공합니다.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)10 X
최종 제품 유형cDNA
반응 수1000 Reactions
최적 반응 온도42°C
제품라인TaqMan
제품 유형MicroRNA Reverse Transcription Kit
수량1,000 reactions
반응 형식Separate Components
역전사 효소MultiScribe™
샘플 종류RNA
배송 조건Dry Ice
크기(최종 제품)200 bp or less
충분1000 Reactions
농도10X
검출 방법Primer-probe
용도(애플리케이션)miRNA analysis
GC-Rich PCR PerformanceHigh
PCR 방법2-step RT-qPCR
반응 속도Fast
Unit SizeEach
구성 및 보관
Contains 10X RT buffer, dNTP mix w/dTTp (100 mM total), RNase inhibitor (20 U/μL), and MultiScribe™ RT enzyme (50 U/μL) for 1000 reactions. Store at -15 to -25°C.

자주 묻는 질문(FAQ)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Can one use the reagents from High Capacity RT kit to make cDNA for TaqMan MicroRNA Assay?

Yes. You can use the reagents from the High Capacity cDNA Kit PN 4368814 (without RNAse Inhibitor) or 4374966 (with RNAse Inhibitor). Make sure to use the reverse transcription primer provided from the TaqMan miRNA assay for the reverse transcription.

Is it possible to combine several miRNA RT primers together for reverse transcription, and if so, how?

Yes. If you are using individual TaqMan MicroRNA Assays, you can prepare your own RT and PreAmp pools. Refer to Protocol for Creating Custom RT and Preamplification Pools using TaqMan MicroRNA Assays (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/4465407_CustomRT-preamp_UG.pdf) for detailed information. Although this protocol has been tested by our development group, we recommend that you validate the performance of the particular pool that you are interested in working with. At a minimum, we recommend running a No Template Control (NTC). The NTC is crucial to identify any primer interactions that may increase the background.

Why does the TaqMan MicroRNA Reverse Transcription Kit (4366597) protocol have an initial 16 degree C step before the reverse transcription step at 42 degrees C?

The 30 min incubation at 16°C allows the RT primer to anneal to miRNA template properly. See the complete protocol at this link.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What concentration of cDNA or range do we recommend for miRNA assays when using the TaqMan MicroRNA Reverse Transcription Kit (Cat. No. 4366596)?

1-10 ng of total RNA.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

View all TaqMan™ MicroRNA Reverse Transcription Kit, 1,000 reactions FAQs