StepOne™ Real-Time PCR System Spectral Calibration Kit (48 well)

사용이 편리한 본 kit는 StepOne™ Real-Time PCR System에서 FAM™/SYBR™ Green I, VIC™/JOE™ 및 ROX™ dye에 대한 순수 dye 스펙트럼과 다양한자세히 알아보기

카탈로그 번호	수량
4371433	1 kit

카탈로그 번호 4371433

제품 가격(KRW)

996,000

카트에 추가하기

1 kit

제품 가격(KRW)

996,000

카트에 추가하기

사용이 편리한 본 kit는 StepOne™ Real-Time PCR System에서 FAM™/SYBR™ Green I, VIC™/JOE™ 및 ROX™ dye에 대한 순수 dye 스펙트럼과 다양한 성분값을 구할 수 있게 합니다.

StepOne™ Real-Time PCR System Spectral Calibration Kit의 특징:

• StepOne™ Real-Time PCR System의 쉽고 빠른 보정을 위해 dye(FAM™/SYBR™ Green I, VIC™/JOE™, ROX™)가 미리 들어있는 48-well 광학 반응 플레이트가 제공됩니다.
• 사용하기 쉽고 반응 혼합물 내 개별 dye의 형광 신호를 정확하게 구분합니다.
• 모든 실시간 및 endpoint 반응에서 신호 정규화를 위해 Passive Reference I standard dye가 포함되어 있습니다.

Applied Biosystems™ StepOne™ Real-Time PCR System Spectral Calibration Kit에는 background 플레이트 1개와 5가지 standard dye가 미리 들어있는 48-well 신속 광학 반응 플레이트 3개가 포함됩니다.

For Research Use Only. Not for use in diagnostic procedures.

보정된 염료FAM, JOE, ROX, VIC, SYBR™ Green

보정 유형Spectral Calibration

검출 방법Fluorescence

용도(애플리케이션)Real Time PCR (qPCR)

용도(장비)StepOne™, Fast Mode, StepOne™, Standard Mode

제품라인StepOne

수량1 kit

배송 조건Dry Ice

제품 유형Spectral Calibration Kit

Unit SizeEach

구성 및 보관

Contains three 48-well reaction plates—one background plate and two containing dye standards(plates I & II). Plate I contains FAM, JOE and ROX; Plate II contains SYBR™ Green I and VIC.

Store at -15 to 25°C. Protect from light.

Expiration date: 12 months from the date of delivery

자주 묻는 질문(FAQ)

What does "Error - Cannot calculate Pure Dye Matrix" mean?

This issue can occur if the run is interrupted. To prevent this from happening in the future, avoid ending the PCR with an infinite hold stage or interrupting the run. Please send the file to techsupport@thermofisher.com so that our software group can attempt to recover the data.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Fatal Error Number 0x8158" mean?

This is generally caused by incorrect placement of tube strips, resulting in a sample block that can't properly be lowered. We recommend contacting Instrument Services to have an engineer walk you through a manual drawer-lowering process during instrument power-up. To prevent this from happening in the future, place tubes toward the middle of the block or use a placeholder strip on the opposing side to counterbalance block pressure.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Java.lang.IllegalArgumentException: One of the raw spectra is null" mean?

This issue can occur if the “Start Run” button is clicked more than once or if illegal characters are used in the experiment/file name. You can recover the data yourself by overriding the calibration file with one that represents the calibration status of the instrument when you ran that particular file. Use the toolbar at the top to navigate: Analysis then Override Calibration then Use Calibration From Another File.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Cannot open the file because file contents are not recognized" mean?

This issue can occur if duplicate samples are added to the experiment, if illegal characters are used in the experiment/file name, or if the file extension was changed to .eds from a different extension.
Please send the file to techsupport@thermofisher.com to attempt recovery.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Analysis failed due to GExSession doesn't exist in context" mean?

This error means that the data collection was not turned on as appropriate. If this is inadvertently turned off, no data will be collected. Thus, the data are not retrievable in such a file.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

View all StepOne™ Real-Time PCR System Spectral Calibration Kit (48 well) FAQs