High-Capacity cDNA Reverse Transcription Kit

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High-Capacity cDNA Reverse Transcription Kit

인용 및 참조 문헌 (1122)

Applied Biosystems™

High-Capacity cDNA Reverse Transcription Kit

Name: High-Capacity cDNA Reverse Transcription Kit
SKU: 4374966
Price: 677000 KRW

High Capacity cDNA Reverse Transcription Kit (구 High Capacity cDNA Archive Kit)는 주입 총 RNA 0.02 ∼2 μg 에서 매우자세히 알아보기

보기 방식 변경

카탈로그 번호	포함	반응 수
4374966	Kit with Rnase Inhibitor	200 Reactions
4368814	Kit only	200 Reactions
4368813	Kit only	1,000 Reactions
4374967	Kit with Rnase Inhibitor	1,000 Reactions

4 개 옵션

카탈로그 번호 4374966

제품 가격(KRW)

677,000

Online offer

Ends: 31-Dec-2025

752,000

할인액 75,000 (10%)

Each

카트에 추가하기

포함:

Kit with Rnase Inhibitor

반응 수:

200 Reactions

제품 가격(KRW)

677,000

Online offer

Ends: 31-Dec-2025

752,000

할인액 75,000 (10%)

Each

카트에 추가하기

High Capacity cDNA Reverse Transcription Kit (구 High Capacity cDNA Archive Kit)는 주입 총 RNA 0.02 ∼2 μg 에서 매우 우수한 single-stranded cDNA를 추출합니다. cDNA 저장소 생성에 이상적임. High Capacity cDNA Reverse Transcription Kit는 최대 2 μg의 input total RNA을 단일 20 μl 반응에 전환시킵니다. 100 μl까지 반응을 확장시켜 단일 반응에서 10 μg cDNA 까지 생성할 수 있습니다. 생성된 cDNA는 real-time PCR, standard PCR, microarray 등 다양한 어플리케이션에 이상적입니다.

• 역동 범위 – 역동 범위 10배
• 민감성 – 0.02 ∼2 μg input total RNA에서 cDNA를 생성합니다.
• 활용성 – 저농도 GC, AU-rich 표적 등 다양한 RNA template과 저농도 발현 RNA에서 시험되었습니다.

이 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.

For Research Use Only. Not for use in diagnostic procedures.

사양

최종 제품 유형First-Strand cDNA

형식Kit

포함Kit with Rnase Inhibitor

반응 수200 Reactions

수량1 Ea.

반응 형식Separate components

시약 유형Reverse Transcription

역전사 효소MultiScribe

리보뉴클레아제 H 활성Yes

배송 조건Dry Ice

크기(최종 제품)7 kb

시작 물질RNA

기술Reverse Transcription

농도50 U/μL

용도(애플리케이션)Real Time PCR (qPCR), RT-PCR

반응 속도120 min.

Unit SizeEach

구성 및 보관

• 10X RT Buffer, 1 mL
• 10X RT Random Primers, 1 mL
• 25X dNTP Mix, 0.2 mL (100 mM)
• MultiScribe Reverse Transcriptase, 2 x 0.1 mL (50 U/μL)
• RNase Inhibitor (1 x 200 μL)

Store at –15 to –25°C.

자주 묻는 질문(FAQ)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Can I order the RNase Inhibitor from the High-Capacity cDNA Reverse Transcription Kit separately?

Yes. You can order the RNase Inhibitor separately as Cat. No. N8080119.

Can one use the reagents from High Capacity RT kit to make cDNA for TaqMan MicroRNA Assay?

Yes. You can use the reagents from the High Capacity cDNA Kit PN 4368814 (without RNAse Inhibitor) or 4374966 (with RNAse Inhibitor). Make sure to use the reverse transcription primer provided from the TaqMan miRNA assay for the reverse transcription.

Which kit should I use for the reverse transcription step for TaqMan Pri-miRNA assays (Cat. No. 4427012)?

We recommend the following kits:

High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4374966).
High-Capacity RNA-to-cDNA Kit (Cat. No. 4387406).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I purchase the 10X RT Buffer from the High-Capacity cDNA Reverse Transcription Kit as a stand-alone item?

Yes, the 10X RT Buffer can be purchased as a stand-alone item (Cat. No. N8080010).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

인용 및 참조 문헌 (1122)

인용 및 참조 문헌

Abstract

Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging.

Authors:Vigetti D,Viola M,Karousou E,Rizzi M,Moretto P,Genasetti A,Clerici M,Hascall VC,De Luca G,Passi A

Journal:The Journal of biological chemistry

PubMed ID:18077444

Increased hepatobiliary clearance of unconjugated thyroxine determines DMP 904-induced alterations in thyroid hormone homeostasis in rats.

Authors:Wong H,Lehman-McKeeman LD,Grubb MF,Grossman SJ,Bhaskaran VM,Solon EG,Shen HS,Gerson RJ,Car BD,Zhao B,Gemzik B

Journal:Toxicological sciences : an official journal of the Society of Toxicology

PubMed ID:15673846

4-(3-pentylamino)-2,7-dimethyl-8-(2-methyl-4-methoxyphenyl)-pyrazolo-[1,5-a]-pyri midine (DMP 904) is a potent and selective antagonist of corticotropin releasing factor receptor-1 (CRF1 receptor) with an efficacious anxiolytic profile in preclinical animal models. In subchronic toxicity studies in Sprague-Dawley rats, DMP 904 produced thyroid follicular cell hypertrophy and hyperplasia, and a low incidence of follicular cell ... More

Calcineurin/nuclear factor of activated T cells and MAPK signaling induce TNF-{alpha} gene expression in pancreatic islet endocrine cells.

Authors:Lawrence MC, Naziruddin B, Levy MF, Jackson A, McGlynn K

Journal:J Biol Chem

PubMed ID:21059644

Cytokines contribute to pancreatic islet inflammation, leading to impaired glucose homeostasis and diabetic diseases. A plethora of data shows that proinflammatory cytokines are produced in pancreatic islets by infiltrating mononuclear immune cells. Here, we show that pancreatic islet alpha cells and beta cells express tumor necrosis factor-alpha (TNF-alpha) and other ... More

TGF-beta regulates miR-206 and miR-29 to control myogenic differentiation through regulation of HDAC4.

Authors:Winbanks CE, Wang B, Beyer C, Koh P, White L, Kantharidis P, Gregorevic P

Journal:J Biol Chem

PubMed ID:21324893

MicroRNAs (miRs) are emerging as prominent players in the regulation of many biological processes, including myogenic commitment and skeletal muscle formation. Members of the TGF-beta family can influence the proliferation and myogenic differentiation of cells, although it is presently not clear what role miRNAs play in the TGF-beta-mediated control of ... More

Somatic cell plasticity and Niemann-Pick type C2 protein: fibroblast activation.

Authors:Csepeggi C, Jiang M, Kojima F, Crofford LJ, Frolov A

Journal:J Biol Chem

PubMed ID:21084287

A growing body of evidence points toward activated fibroblasts, also known as myofibroblasts, as one of the leading mediators in several major human pathologies including proliferative fibrotic disorders, invasive tumor growth, rheumatoid arthritis, and atherosclerosis. Niemann-Pick Type C2 (NPC2) protein has been recently identified as a product of the second ... More

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