Fast SYBR™ Green Master Mix
Product Image
Applied Biosystems™

Fast SYBR™ Green Master Mix

Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for자세히 알아보기
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호수량
43856101 x 1 mL
43856121 x 5 mL
43856162 x 5 mL
43856175 x 5 mL
438561810 x 5 mL
43856141 x 50 mL
카탈로그 번호 4385610
제품 가격(KRW)
122,000
Each
카트에 추가하기
수량:
1 x 1 mL
제품 가격(KRW)
122,000
Each
카트에 추가하기
Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR™ Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we've taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
형식Tube
반응 수100 Reactions
패시브 참조 염료ROX (Pre-mixed)
중합효소AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
제품라인SYBR
제품 유형Real Time PCR SYBR Master Mix
순도 또는 품질 등급UP (Ultra Pure)
수량1 x 1 mL
샘플 종류DNA (Genomic), cDNA
배송 조건Dry Ice
충분100 Reactions of 20 μL
부피1 mL
농도2X
검출 방법SYBR
용도(애플리케이션)Gene Expression
GC-Rich PCR PerformanceHigh
라벨 또는 염료SYBR Green
PCR 방법qPCR
반응 속도Fast
Unit SizeEach
구성 및 보관
1 x 1 mL vial. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1 and optimized buffer components. 100 reactions in a 20 μL total volume. Store at -5 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

자주 묻는 질문(FAQ)

Is there a SYBR Green Fast Master Mix currently available?

Yes. The following reagents can be used for fast cycling: Fast SYBR Green Master Mix, EXPRESS SYBR GreenER qPCR Supermix Universal, and SYBR GreenER qPCR SuperMix for ABI PRISM instruments.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.
Authors:Hulsmans M, Van Dooren E, Mathieu C, Holvoet P,
Journal:PLoS One
PubMed ID:22393448
Low adiponectin, a well-recognized antidiabetic adipokine, has been associated with obesity-related inflammation, oxidative stress and insulin resistance. Globular adiponectin is an important regulator of the interleukin-1 receptor-associated kinase (IRAK)/NF?B pathway in monocytes of obese subjects. It protects against inflammation and oxidative stress by inducing IRAK3. microRNA (miR)-146b-5p inhibits NF?B-mediated inflammation ... More